| 时 涛，李超萍，蔡吉苗，李博勋，黄贵修.一个预测的木薯衰老相关基因的
获得及其功能分析[J].广东农业科学,2017,44(2):6-11 |
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一个预测的木薯衰老相关基因的
获得及其功能分析 |
| Obtaining and function analysis of predictedsenescence-associated gene from cassava |
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| DOI：10.16768/j.issn.1004-874X.2017.02.002 |
| 中文关键词: 木薯 衰老相关基因 功能分析 |
| 英文关键词: cassava senescence-associated gene function analysis |
| 基金项目:农业部现代农业人才支撑计划项目（0316001）；国家木薯产业技术体系建设项目（CARS-12-
hnhgx）；海南省自然科学基金（20153046） |
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| 摘要点击次数: 2250 |
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| 中文摘要: |
| 根据水稻LRR 类抗病基因Xa21 的保守结构域设计1 对简并引物，提取木薯抗/ 感细菌性萎
蔫病种质E1340 和GR911 的基因组DNA 为模板，通过PCR 扩增均获得大小约0.5 kb 的扩增片段。两个片
段克隆、测序后获得完全一致的474 nt 序列，比对发现木薯种质AM560 带有与该序列高度同源的核苷酸片
段。提取包括同源片段上下游各约2.2 kb 的大片段序列进行基因预测，发现该片段位于1 个预测基因内，命
名为SLR1。该预测基因ORF 全长1 641 nt，编码546 aa，与已报道的衰老相关蛋白具有较高的同源性，可能
在木薯衰老相关反应中发挥重要作用。 |
| 英文摘要: |
| One pair of degenerated primers was designed based on the conserved domain of resistance gene
Xa21，which had LRR-liked domain and came from rice. The genome DNA of cassava germplasm E1340 and GR911
was extracted and used as template，which was resistant and susceptible to cassava bacterial blight separately. Two
products of about 0.5 kb were obtained by PCR reaction. These two products were purified，cloned and sequenced，
and then the same sequences of 474 nt were got. This sequence was highly matched with the homology segment
anchored on the genome of cassava germplasm AM560. The long segment including each about 2.2 kb sequences from
upstream and downstream of the homology segment was obtained，then one gene was predicted and named SLR1.
Sequence analysis results of SLR1 showed that the ORF included 1 641 nucleotides encoding 546 amino acids. This
predicted protein was higher consistent with senescence-associated protein reported，may play an important role in
senescence mechanism of cassava. |
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