| 邓奥宇1，关统伟1，王鹏昊1，李智强2，向慧平1，赵顺先1，张习超1.堆肥中木质素降解细菌MZ-9 的筛选
及产酶条件优化[J].广东农业科学,2017,44(2):95-103 |
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堆肥中木质素降解细菌MZ-9 的筛选
及产酶条件优化 |
| Screening of lignin degradation strain MZ-9 and optimizationof enzyme production conditions in compost |
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| DOI：10.16768/j.issn.1004-874X.2017.02.015 |
| 中文关键词: 堆肥 地衣芽孢杆菌 漆酶（Lac）活性 锰过氧化物酶（MnP）活性 木质素 生物降解 |
| 英文关键词: compost Bacillus licheniformis Laccase（ Lac） activity Manganese Peroxidase（ MnP） activity lignin biodegradation |
| 基金项目:成都市农业技术研发项目（2015-NY02-00007-NC）；成都市高校人才创新服务项目（2015- RC03-
00033-HZ）；成都市产学研联合实验室项目（2015-YF04-00052-JH，15205206） |
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| 中文摘要: |
| 在堆肥中依次采用铁氰化钾显色、愈创木酚显色、苯胺蓝（Azure-B）退色圈试验以及液体发
酵验证试验测得漆酶（Lac）、锰过氧化物酶（MnP）的活性分别高达9.53 U/mL、21.56 U/mL，木质素降解
率达20.32%，从而筛选到一株高活性木质素降解菌株MZ-9。生理生化试验及16S rDNA 序列分析表明，菌
株MZ-9 属于芽孢杆菌属（Bacillus），与地衣芽孢杆菌（Bacillus licheniformis）相似性为99.2%。产酶条件
试验表明，当pH 值8.0、培养温度37℃、接种量8%、培养时间6 d 时，菌株MZ-9 的Lac、MnP 活性最高，
此时木质素降解率为20.51%。因此，菌株MZ-9 是一株优良的木质素降解菌株，在木质素降解方面具有很
好的开发利用价值，可为后续的工业化生产提供可靠的菌种资源支撑。 |
| 英文摘要: |
| This experiment use the potassium ferricyanide color，guaiacol color and aniline blue（ Azure -
B） fading circle method，and liquid fermentation experiment，measured enzyme activitives of Laccase（ Lac） and
Manganese Peroxidase（ MnP） were as high as 9.53 U/mL，9.53 U/mL，lignin degradation rate was 20.32%，from the
compost. An active strain MZ-9 is screened as a strong lignin degradation strain. Physiological and biochemical tests
and 16S rDNA sequence analysis showed that strain MZ-9 belonged to Bacillus and had 99.2% similar to Bacillus
licheniformis. Enzyme production showed that when the pH value was 8.0，the culture temperature was 37℃，
the inoculation amount was 8% and the culture time was 6 d，the Lac and MnP activities of strain MZ-9 were the
highest，and the degradation rate of lignin was 20.51%. Therefore，strain MZ-9 is an excellent lignin degradation
strain，has a very good development and utilization value in the lignin degradation，and can provide a reliable strain
resource support for the subsequent industrial production. |
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