| 郑 丽1，2，张海鹏2，宋艳培3，刘孟浩3，何时雨1，马旭东1，覃新导4，李伯松1.纤维素降解菌的筛选、鉴定和糖化水平研究[J].广东农业科学,2017,44(2):104-111 |
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纤维素降解菌的筛选、鉴定和糖化水平研究 |
| Isolation，identification and saccharificationlevel of cellulose degrading bacteria |
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| DOI：10.16768/j.issn.1004-874X.2017.02.016 |
| 中文关键词: 细菌 纤维素酶 糖化水平 |
| 英文关键词: bacteria cellulase saccharification level |
| 基金项目:中国热带农业科学院院本级基本科研业务费专项资金（1630052015047）；海南省自然科学基金
（314185）；广东省科技计划项目（2015A020209027） |
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| 中文摘要: |
| 将前期从木薯生境中分离、保存的298 个菌株，通过平板测试，筛选到57 个可产纤维素酶活
的菌株，其中酶活性效果最好的菌株（水解圈最大，将其赋值得分为3）所占比例为40.9%，酶活性依次减
弱的菌株所占比例（赋值得分为2 和1）分别占27.9% 和24.6%。采用DNS 法测定57 个菌株的糖化水平
和酶活力，结果显示，10 株细菌具有较好潜力降解纤维素，其中菌株HWY-3-9 的糖化和酶活力最好，产糖
量为0.1852 mg/mL，酶活力为0.0012 U/mL，经鉴定为解淀粉芽孢杆菌。 |
| 英文摘要: |
| In this study，298 bacteria were isolated from cassava microenvironments and 57 strains with
cellulase activity were gained by in vitro detection test. Results showed that strains with big hydrolysis circle (the
score of 3) accounted for 40.9% and strains with weakly hydrolysis circle（the score of 2 and 1） accounted for
27.9% and 24.6%，respectively. The saccharification level and enzyme activity were tested by DNS method，and we
found 10 potential strains with efficient ability of degradation of cellulose，in which strain HWY-3-9 had the best
saccharification and enzyme activity， the sugar content was 0.1852 mg/mL and enzyme activity was 0.0012 U/mL，
and it was identified as Bacillus amyloliquefaciens. |
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