| 付瑜华，贺尔奇，雷石富，李向勇，张正学，卢加举.2 种甘蔗病害胁迫果蔗NBS 类抗病基因
同源序列的表达分析[J].广东农业科学,2017,44(2):118-123 |
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2 种甘蔗病害胁迫果蔗NBS 类抗病基因
同源序列的表达分析 |
| Expression analysis of NBS resistance gene analogs fromchewing cane under two sugarcane diseases stress |
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| DOI：10.16768/j.issn.1004-874X.2017.02.018 |
| 中文关键词: 果蔗 眼斑病 轮斑病 抗病基因同源序列 表达分析 |
| 英文关键词: chewing cane Drechslera sacchari（ Butler） Subram. and Jain Leptosphaeria sacchari Breda de
Haan resistance gene anologs expression analysis |
| 基金项目:贵州省科技厅联合基金（黔科合J 字LKN[2013]03 号）；贵州省亚热带作物科技创新人才基地建设经
费（黔人领发[2016]22 号） |
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| 中文摘要: |
| 在眼斑病和轮斑病病原菌侵染下，分析果蔗NBS 类抗病基因同源序列在侵染初期的表达情
况，为后续克隆关键抗病基因、研究抗病机理提供理论依据。已克隆的7 条果蔗RGAs 可视为来源不同的
3 个基因片段。将2 种病原菌分别针刺接种到感病基因型黔糖3 号和抗病基因型黔糖5 号叶片后，3 条果
蔗RGAs 在2 种病害侵染初期均受到不同程度的上调，RGA12478 对两种病害的响应最为迅速，RGA5 的
表达量最高，尤其在轮斑病菌的胁迫下，RGA5 在胁迫后12 h 的表达量大约分别是同时间点RGA12478 和
RGA36 表达量的16 倍和6 倍。因此，RGA5 可认为是果蔗在抵御轮斑病和眼斑病侵染初期发挥主要作用
的抗病基因片段，后续应结合RACE 技术获得基因全长，作为关键抗病候选基因研究其功能和在外源信号
因子作用下的表达情况，全面了解该抗病基因的抗病机理。 |
| 英文摘要: |
| Expression analysis of NBS resistance gene anologs（ RGA） from chewing cane under infection
of Drechslera sacchari（ Butler） Subram. and Jain and Leptosphaeria sacchari Breda de Haan，respectively，were
performed in order to provide theoretical basis for cloning disease resistance genes and studying disease resistance
mechanism. seven cloned chewing cane RGAs were considered as 3 gene fragments. The 2 pathogenic bacteria
were inoculated into leaves of the susceptible genotype QT 3 and the disease resistant genotype QT 5 using needle
puncturing method respectively，all 3 chewing cane RGAs were up-regulated in the initial infection. The response
of RGA12478 to the two diseases was the most rapid while the expression of RGA5 was the highest. Especially
under the infection of L. sacchari，the expression of RGA12478 at 12h was about 16 and 6 times of RGA5 and
RGA36 expression at the same time point respectively. Therefore，RGA5 was considered to be a key disease
resistance gene fragment from chewing cane playing a major role in resisting the infection of D. sacchari and L.
sacchari in the initial infection stage respectively. To study the overall disease resistance mechanism of RGA5，
follow-up research should includ cloning the full-length gene using RACE technology，analysis of gene function
and expression level under exogenous signal factor treatments. |
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