| 洪宇航.溴氰菊酯对中华绒螯蟹主要免疫指标的影响[J].广东农业科学,2017,44(3):151-157 |
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溴氰菊酯对中华绒螯蟹主要免疫指标的影响 |
| Effects of deltamethrin on main immune parametersof Chinese mitten crab，Eriochier sinensis |
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| DOI：10.16768/j.issn.1004-874X.2017.03.022 |
| 中文关键词: 溴氰菊酯 中华绒螯蟹 免疫功能 毒性作用 |
| 英文关键词: deltamethrin Eriochier sinensis immunity function toxicity |
| 基金项目:四川省教育厅科研一般项目（17ZB0405） |
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| 中文摘要: |
| 为揭示溴氰菊酯对中华绒螯蟹免疫功能的影响，测定了在不同浓度溴氰菊酯（0、0.19、0.30、
0.41、0.52 μg/L）条件下中华绒螯蟹总血细胞数量（THC）、吞噬活性、酚氧化酶（PO）活性、酸性磷酸酶
（ACP）活性和碱性磷酸酶（AKP）活性5 项主要免疫指标的变化情况。结果表明，溴氰菊酯对中华绒螯
蟹免疫有显著影响，低浓度处理（0.19、0.30 μg/L）引起THC 药后6 h 和12 h 出现极显著上升，随后逐渐
恢复；而高浓度处理（0.41、0.52 μg/L）则在药后24 h 后持续下降，药后96 h 极显著低于对照。各浓度处
理均对血细胞吞噬活性有抑制效应，且随着浓度升高抑制效果越强。0.52 μg/L 处理PO 活性药后6 h 出现
极显著上升，随后逐渐下降，药后96 h 处理间无显著差异；而ACP 活性、AKP 活性药后6 h 出现明显下降，
各时间段均极显著低于对照。 |
| 英文摘要: |
| In this research，the effects of deltamethrin on immune parameters were revealed by dectecting the
changes of total hemocytes count（ THC），phagocytic activity，phenoloxidase（PO） activity，acid phosphatase
（ACP） activity and alkaline phosphatase（AKP） activity in hemolymph of Chinese mitten crab under different
concentrations of deltamethrin（0，0.19，0.30，0.41，0.52 μg/L） exposure. The results showed that deltamethrin
had evident effects on immune parameters of Chinese mitten crab，THC increased after 6 h and 12 h exposure to low
concentrations of deltamethrin including 0.19 μg/L and 0.30 μg/L，and then decreased to normal level at 96 h. On the
contrary，high concentration（ 0.41μg/L and 0.52μg/L） exposure declined the THC after 24 h，which was significantly
lower than that of control group at 96 h. The phagocytic activity decreased under each concentration and the higher
concentration made a higher inhibition of phagocytic activity. After exposure to 0.52μg/L deltamethrin，PO activity
increased significantly at 6 h and then decreased gradually till 96 h，which had no significant difference among
each group. However，both ACP and AKP activity decreased at 6 h exposure to 0.52μg/L deltamethrin and was
significantly lower than those of control at each time point. |
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