| 于林洋1，董建国1，2，张乐宜1，刘燕玲1，梁鹏帅1，王 磊1，宋长绪1.猪繁殖与呼吸系统综合征病毒华南株GDgz
的分离鉴定及遗传进化分析[J].广东农业科学,2017,44(4):138-145 |
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猪繁殖与呼吸系统综合征病毒华南株GDgz
的分离鉴定及遗传进化分析 |
| Isolation，identification and genetic variation analysisof South China strain GDgz of porcine reproductiveand respiratory syndrome virus |
| |
| DOI：10.16768/j.issn.1004-874X.2017.04.021 |
| 中文关键词: 猪 繁殖与呼吸综合征病毒 分离鉴定 遗传进化 |
| 英文关键词: porcine reproductive and respiratory syndrome virus isolation and identification genetic
variation |
| 基金项目:国家科技支撑计划项目（2015BAD12B02-5） |
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| 摘要点击次数: 2638 |
| 全文下载次数: 1021 |
| 中文摘要: |
| 为了解华南地区猪繁殖与呼吸综合征病毒（PRRSV）流行株的变异特性，用Marc-145 细胞
从广东省疑似猪繁殖与呼吸综合征发病猪场采集的肺组织中分离得到1 株PRRSV（命名为GDgz），该毒
株能产生明显的细胞病变。全基因组进化分析和同源性比对分析结果显示，GDgz 与中国高致病性毒株位
于同一分支，与欧洲型毒株Lelystad-virus 同源性最低、仅为60.3%，与美洲型经典毒株VR-2332 的同源
性为88.8%，与中国高致病性毒株JXA1 和HuN4 同源性为98.8% 和98.9%，与JXA1 的疫苗株同源性为
91.1%，与NADC30 和NADC30-like 毒株 CHsx1401 和JL580 同源性分别为84.5%、87.2% 和83.6%。NSP2
序列比对结果显示，GDgz 在高变区存在30 个不连续氨基酸缺失。GP5 序列比对结果显示，GDgz 在GP5
抗原表位上有氨基酸突变，表明GDgz 属于美洲型高致病性毒株传代致弱的疫苗株，且在抗原表位上有一
定程度的突变。 |
| 英文摘要: |
| To understand the evolution of porcine reproductive and respiratory syndrome virus（PRRSV）
in South China，we isolated a PRRSV strain in Marc-145 cell from lung of suspected PRRSV-positive pig in South
China and named GDgz. GDgz strain could cause typical cenotaphic effect. The results of phylogenetic tree and
homology alignment analysis showed that GDgz belonged to the same cluster with high pathogenetic PRRSV（HPPRRSV）
strains in China；GDgz had a far distance with European strain Lelystad virus，the nucleotide homology was
only 60.3%，with American classic strain VR-2332 was 88.8%，with Chinese HP-PRRSV strains JXA1 and HuN4
were 98.8% and 98.9%，with the vaccine strain JXA1-P80 of JXA1 was 99.1%，with NADC30 and NADC30-like
strains CHsx1401 and JL580 were 84.5%，87.2 and 83.6%，respectively. The result of sequence alignment showed that there were 30 discontinuous amino acids deletion in the high variable region of NSP2. These results showed that
GDgz belonged to vaccine strain of high pathogenetic North American type PRRSV and had some mutations in the
epitope region. |
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