| 黄燕琼1，张 森2，何淑华1，姚静雯1，戴 金1，邓 艳1，柏建山1.广州白云机场口岸入境海鱼感染
异尖线虫鉴定研究[J].广东农业科学,2017,44(4):146-151 |
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广州白云机场口岸入境海鱼感染
异尖线虫鉴定研究 |
| Identification on the infection of Anisakis frommarine fishes imported by Baiyun Airport |
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| DOI：10.16768/j.issn.1004-874X.2017.04.022 |
| 中文关键词: 异尖线虫 形态学鉴定 PCR 扩增 序列分析 |
| 英文关键词: Anisakis morphological identification PCR amplification sequence analysis |
| 基金项目:广东省科技厅科技专项（2014A040401063） |
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| 中文摘要: |
| 对2012—2015 年从白云机场口岸入境的214 批海鱼进行剖检鉴定异尖线虫，取内脏和鱼肉
进行酶消化，在10 批次海鱼中分离出虫体，用苯酚乳酸透明液进行透明处理及形态学鉴定，确定所分离
出的虫体有异尖线虫三期幼虫I 型和Ⅱ型。用线虫rDNA ITS 区通用引物NC5 和NC2 对虫体进行PCR 扩
增，并对虫体的核糖体基因间隔区ITS1 和ITS2 进行序列和进化分析，确定分离出来的异尖线虫三期幼虫
包括简单异尖线虫（Anisakia simple）、抹香鲸异尖线虫（Anisakia physeteris）和典型异尖线虫（Anisakia
typica）。调查结果对公共安全的风险评估有重要意义，同时为口岸的进境水产品食品安全风险评估提供参
考数据。 |
| 英文摘要: |
| 214 batches of marine fish imported in 2012-2015 from Baiyun Airport in Guangzhou were examined
for Anisakis larvae by dissection and digest. 10 batches of Anisakis larvae were isolated from marine fishes by
enzymatic degradation. Some Anisakis larvae isolated from imported marine fish were identified via morphology as the
third larvae of Anisakis Type I and the rest were identified as the third larvae of Anisakis Type Ⅱ . Sequencing and
phylogenetic analysis of the ribosomal internal transcribed spacer（ ITS） gene identified nematodes as A. simplex，A.
physeteris and A. typica. The study has an important impact on the public health risk assessment，and also provides
the reference for food safety risk assessment of imported marine fish and aquatic products. |
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