| 李丽梅1，张拜宏1，李晓云1，徐温丽1，李 玲1，李海芬2.ELISA 法检测干旱下花生不同品种中AhNCED1 蛋白表达变化[J].广东农业科学,2017,44(5):7-12 |
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ELISA 法检测干旱下花生不同品种中AhNCED1 蛋白表达变化 |
| AhNCED1 protein expression in different peanut varieties in response to drought by ELISA |
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| DOI：10.16768/j.issn.1004-874X.2017.05.002 |
| 中文关键词: ELISA 花生 抗旱性 AhNCED1 |
| 英文关键词: ELISA peanut drought resistance AhNCED1 |
| 基金项目:广东省科技计划项目（2013B020301004） |
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| 摘要点击次数: 2052 |
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| 中文摘要: |
| ABA 合成关键酶AhNCED1 与花生抗旱性相关。优化ELISA 法检测花生AhNCED1 蛋白体系，
利用该体系检测不同产地7 个花生品种幼苗干旱处理24 h 后叶片内AhNCED1 蛋白表达变化。结果表明，
正常生长条件下，7 个品种AhNCED1 蛋白表达量呈现较低水平，品种间无显著性差异；干旱24 h 后，所有
品种的AhNCED1 蛋白表达量显著增加；AhNCED1 蛋白表达变化倍数从大到小依次是：中花16 > 濮花28 >
花育19 > 福花4 > 中花6 > 花育33 > 海花1，与QPCR 检测AhNCED1 基因表达和Western blot 的检测结果
比较一致。综合分析3 种方法检测不同品种的AhNCED1 基因和蛋白结果表明，ELISA 法及QPCR 配合使用
适用于大范围的快速筛选抗旱花生品种，为抗旱花生品种筛选提供技术支撑。 |
| 英文摘要: |
| AhNCED1，the rate-limiting enzyme in the ABA biosynthetic pathway was found to be related with
peanut drought resistance. The ELISA detection system was optimized to analyze the expression of AhNCED1 protein.
The expression of AhNCED1 in leaves of 7 different peanut varieties under drought stress of 24 h was detected by
ELISA system. No significant difference was found among different peanut varieties under normal condition. On the
contrary，the expression of AhNCED1 proteins of seven peanut varieties remarkably increased under drought stress.
The expression change of AhNCED1 of peanut varieties was Zhonghua 16> Puhua 28>Huayu 19>Fuhua 4>Zhonghua
6>Huayu 33>Haihua 1，respectively，which was consistent with the results of QPCR and WB. The ELISA and QPCR
for the detecting AhNCED1 expression in different peanut varieties were recommended for selection of droughtresistant
peanut varieties rapidly and largely，providing technology support for screening peanut varieties with drought
resistant. |
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