| 陈月桂，谭嘉娜，官锦燕，罗剑飘，黄海英，罗青文，杨俊贤，李奇伟.甘蔗新品种粤糖03-373 组培快繁试验[J].广东农业科学,2017,44(5):13-18 |
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甘蔗新品种粤糖03-373 组培快繁试验 |
| Tissue culture and rapid propagation of Yuetang 03-373，a new sugarcane variety |
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| DOI：10.16768/j.issn.1004-874X.2017.05.003 |
| 中文关键词: 甘蔗 粤糖03-373 组培快繁 愈伤组织培养 茎尖和腋芽培养 |
| 英文关键词: sugarcane Yuetang 03-373 tissue culture and rapid propagation callus culture shoot-tip
and axillary bud culture |
| 基金项目:广东省科技计划- 科技基础条件建设项目（2015A030302009）；湛江市科技计划项目（2015A03014） |
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| 中文摘要: |
| 以粤糖新品种03-373 的成熟甘蔗种茎作为研究材料，研究不同植物生长调节剂配比对愈伤
组织脱分化和再分化诱导、茎尖与腋芽萌发诱导、丛芽增殖培养、生根培养的影响。结果表明，适合粤糖
03-373 的最佳综合组培方案为：诱导愈伤组织脱分化适宜培养基为MS+2，4-D1.0 mg/L，诱导愈伤组织再
分化的适宜培养基为MS+BA 0.5 mg/L +IBA 0.01 mg/L，诱导茎尖与腋芽萌发的适宜培养基为MS+ BA 1.0
mg/L + IBA 0.04 mg/L，丛芽继代增殖适宜培养基为MS+ BA 1.0 mg/L + IBA 0.04 mg/L，生根培养基以1/2MS
+ ABT 0.20 g/L + IBA 0.05 mg/L + 活性碳0.5 g/L；继代培养基中添加不同浓度椰汁、水解酪蛋白和酵母提取
物，组培苗黄化率由低到高依次为椰汁＜水解酪蛋白＜酵母提取物＜对照；采用简易的沙培方式，组培苗
的假植成活率达99％以上，大田移栽成活率达98％。 |
| 英文摘要: |
| Experiments were carried out using stem of a new sugarcane variety Yuetang 03-373 as
materials，the best synthetic way of tissure culture was obtained by different exogenous hormones ratio for inducing
dedifferentiation and redifferentiation of callus，inducing germination of shoot-tip and axillary bud，clustered buds
cultivation，multiplication during subculture and rooting. The results showed that for Yuetang 03-373，MS + 2，
4-D mg/L was the optimal medium for callus induction，MS+BA0.5 mg/L + IBA0.01 mg/L was the optimal medium
for buds differentiation from callus induction，MS+ BA1.0 mg/L + IBA 0.04 mg/L was the optimal medium for
germination of shoot-tip and axillary bud，MS + BA1.0 mg/L +IBA0.04 mg/L was the optimal proliferation medium，
and the best medium for rooting was 1/2MS + ABT0.20 g/L + IBA0.05 mg/L + Ac0.5 g/L . Etiolation rates of plants
were in order from low to high among coconut juice，casein hydrolysate，yeast extract and control in the subculture
medium . The survival percentage of transplant was up to 99% with the simpal sand medium，transplanting survival
rate was up to 98% in the soil. |
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