| 李桂花，陈汉才，张 艳，黎庭耀.小白菜种质遗传多样性与亲缘关系的SRAP 和SSR 分析[J].广东农业科学,2017,44(5):37-45 |
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小白菜种质遗传多样性与亲缘关系的SRAP 和SSR 分析 |
| Genetic diversity and phylogenetic relationships analysis of Chinese cabbage germplasm resourcesby SRAP and SSR… |
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| DOI：10.16768/j.issn.1004-874X.2017.05.007 |
| 中文关键词: 小白菜 SRAP SSR 聚类分析 遗传多样性 |
| 英文关键词: Chinese cabbage SRAP SSR cluster analysis genetic diversity |
| 基金项目:广东省科技计划项目（2014A020208042）；广州市科技计划项目（201508020004） |
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| 中文摘要: |
| 利用SRAP 和SSR 标记对小白菜进行遗传多样性分析，从666 对SRAP、160 对SSR 引物中筛
选出59 对多态性明显、条带清晰、稳定可靠的引物，对41 份小白菜材料进行扩增，共扩增出519 条带，平
均每对引物扩增出6.2 条，扩增出多态性条带数171 条，多态性比例为32.9%。利用聚类软件进行分析，41
份小白菜种间遗传距离在0.58~0.87 之间，在遗传相似系数0.58 处可将41 份小白菜材料分为两大类。研究
表明，小白菜品种具有丰富的遗传多样性，大多数小白菜种质资源之间的亲缘关系与其地理来源有较大的
相关性。SRAP 标记适用于分析种质的遗传距离，从种质资源保存的角度分析，SSR 标记能够较全面地保证
种质资源遗传多样性。 |
| 英文摘要: |
| SRAP and SSR makers were used to analyze the genetic diversity of Chinese cabbage. 59 pairs
of SRAP and SSR primers were selected from 666 SRAP and 160 SSR primers，which could produce stable and
polymorphic bands. 519 bands were amplified from 41 samples and each primer resulted in 6.2 bands. 171 bands
were polymorphic（ percentage of polymorphic bands was 32.9%）. The value of Nei’s genetic variations indexes of
41 cultivars based on the SRAP and SSR data with the cluster analysis varied from 0.58 to 0.87 and the cultivars could
be divided into two groups. The results showed that Chinese cabbage varieties owned abundant genetic diversities
and most of them were clustered based on the source of cultivars. The investigation showed that SRAP was suitable
for analyzing the genetic distance，while for conserving breeding resources，SSR would be a better choice to ensure
genetic diversity. This study combined with SRAP and SSR markers for the first time to analyze the genetic diversity of
Chinese cabbage，which would play an important role in Chinese cabbage breeding. |
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