| 李 诺1，孔倩莹2，宾金华2.花生种子萌发过程中子叶内肽酶活性研究[J].广东农业科学,2017,44(6):1-7 |
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花生种子萌发过程中子叶内肽酶活性研究 |
| Study on endopeptidase activity during peanut seeds germination |
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| DOI：10.16768/j.issn.1004-874X.2017.06.001 |
| 中文关键词: 花生 种子萌发 内肽酶 同工酶 种子活力 子叶 |
| 英文关键词: Arachis hypogaea L. seed germination endopeptidase isoenzyme seed vitality cotyledon |
| 基金项目:广东省高等院校学科建设和教学质量与教学改革工程项目（415B0208） |
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| 摘要点击次数: 2446 |
| 全文下载次数: 785 |
| 中文摘要: |
| 探讨花生种子在萌发过程中子叶内肽酶的活性及其同工酶数量的变化，研究金属型蛋白酶抑
制剂EDTA 与不同温度对内肽酶的活性的影响。采用直立玻板法萌发花生种子，运用分光光度法测定内肽
酶的活力，结果发现内肽酶活力在种子萌发6 d 达到最大值；运用SDS-PAGE 凝胶电泳法发现在种子萌发
6 d 出现5 条内肽酶同工酶（其中2 条是金属型内肽酶）；在种子萌发8 d 和10 d 均出现7 条内肽酶同工
酶（其中4 条是金属型内肽酶），这些内肽酶同工酶的最适温度约为40℃，EGTA 对萌发6 d 时存在的2
种金属型内肽酶同工酶有明显抑制作用，但并不能完全抑制其活性。 |
| 英文摘要: |
| The activity and isozyme of endopeptidases during peanut seeds germination were detected to
research the activity of endopeptidase affected by different temperatures and inhibitors of metalloprotease EDTA.
In this study，we used the standing boards to germinate peanut seeds，used spectrophotometry to determine the
activity of endopeptidase during the germination of seeds. It was found that the ativity of endopeptidase reached the
maximum at the sixth day. Using SDS-PAGE gel electrophoresis，we found 5 kinds of endopeptidase isoenzymes，
in which there were two kind of metalloprotease of the 6 days of germination，and 7 kinds of endopeptidase
isoenzymes，which there were 4 kind of metalloprotease after 8 days and 10 days of germination. The optimum
temperature of these endopeptidase isozymes was about 40℃. Gelatin electrophoresis results showed that EGTA
had inhibitory effect on two isozymes of metal type at 6 days of germination，but it did not completely inhibit the
activity. |
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