| 王纪坤，王立丰，安 锋，谢贵水.巴西橡胶树逆境响应基因HbPRX53 的克隆与表达分析[J].广东农业科学,2017,44(6):63-70 |
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巴西橡胶树逆境响应基因HbPRX53 的克隆与表达分析 |
| Cloning and gene expressions of stress responsive gene HbPRX53 in rubber tree |
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| DOI：10.16768/j.issn.1004-874X.2017.06.009 |
| 中文关键词: 干旱 HbPRX53 橡胶树 激素 |
| 英文关键词: drought HbPRX53 rubber tree hormone |
| 基金项目:海南省自然科学基金（20153151）；国家现代农业产业技术体系建设专项（CARS-34-ZP1）；中国热
带农业科学院橡胶研究所基本科研业务费专项（1630022015013） |
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| 中文摘要: |
| 在干旱胁迫下，橡胶树过氧化物酶活性显著增加。为了研究干旱响应的过氧化物酶基因功
能，从橡胶树中克隆了HbPRX53 全长DNA。生物信息学分析结果表明，HbPRX53 含有334 个氨基酸和
1 个植物过氧化物酶结构域。聚类分析结果发现，其与拟南芥过氧化物酶AtPRX53 最相近。HbPRX53 在
橡胶树树皮、叶片、胶乳和花中均有表达，但在叶片中表达量最高，白粉菌侵染、机械伤害和干旱显著
上调叶片中HbPRX53 的表达。此外，吲哚乙酸、脱落酸、水杨酸乙烯、过氧化氢和茉莉酸处理也会上调
HbPRX53 表达。这说明HbPRX53 与橡胶树对生物和非生物胁迫的响应密切相关。 |
| 英文摘要: |
| Peroxidase activity significantly increased under drought stress in rubber tree. To identify the
functions of peroxidase genes responsive to drought stress，the full-length cDNA of HbPRX53 was isolated
from rubber tree. Bioinformatics analysis showed that HbPRX53 contained 334 amino acid residues and a plant
peroxidase-like superfamily domain. Phylogenetic analysis with Arabidopsis Class III peroxidases revealed that
HbPRX53 shared high identities with AtPRX53. Although HbPRX53 was expressed in bark，leaf，latex and
flower，it was preferentially expressed in leaf in rubber tree. HbPRX53 expression was significantly upregulated
in leaves by powdery mildew infection，mechanical wounding and drought stress. Moreover，indole acetic acid，
abscisic acid，salicylic acid ethylene，H2O2 and methyl jasmonate treatments also led to marked accumulation of
HbPRX53 transcripts in leaves. These results indicated HbPRX53 is a key factor in both biotic and abiotic stress
responses in rubber tree. |
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