郑 丽1,2,3,张海鹏3,林 江4,王娇娇4,李梦倩4,潘登浪1,李 静2,曾宪海1.24 个油棕品种对3 种真菌性叶斑病的抗病性评价及生防细菌的分离、筛选[J].广东农业科学,2017,44(7):96-103 |
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24 个油棕品种对3 种真菌性叶斑病的抗病性评价及生防细菌的分离、筛选 |
Resistance evaluation of 24 oil palm species to three fungal pathogens and the isolation and screeningof biological control agents |
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DOI:10.16768/j.issn.1004-874X.2017.07.016 |
中文关键词: 油棕 抗性评价 颉颃 水解酶 筛选 |
英文关键词: oil palm resistance evaluation antagonistic hydrolytic enzymes screening |
基金项目:中国热带农业科学院橡胶研究所重点实验室开放课题(RRI-KLOF201508);农业部热作种质资源保护
项目(151721301354052014);中国热带农业科学院基本科研业务费专项(1630112017002) |
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中文摘要: |
通过针刺接种方法,将24 个油棕品种对3 种病原菌(YZ-4、YZ-6 和 YZ-8)的抗病性进行
评价,发现对YZ-4 抗病性较强的油棕品种为RY-2 和RY-6,对YZ-6 抗病性较强的油棕品种为RY-9、
RY-14、RY-15、RY-31、RY-37、RY-38,对YZ-8 抗病性较强的油棕品种为RY-38;可见不同品种对病原菌
的抗性存在差异。针对3 种病原菌进行生防菌株的分离和筛选,从不同生境(根、叶、土壤)中分离到138
株细菌,测定其几丁质酶、蛋白酶、纤维素酶、葡聚糖酶活性,以及产生吲哚乙酸和嗜铁素等次生代谢物能
力,采用平板对峙法进行颉颃活性测定。结果表明,138 株菌株产生长素的菌株最多,比例达75.36%;无产
几丁质酶和葡聚糖酶的菌株;产蛋白酶、纤维素酶和嗜铁素的比例分别为67.39%、58.70% 和63.04%。AR156
对YZ-4 具有很强颉颃性,综合赋值总分为13;HNLE-4-2 和3BS3 对YZ-6 具有最好颉颃效果,其综合赋值
总分为10;3BS3 对YZ-8 具有最好的颉颃效果,综合赋值总分为10。 |
英文摘要: |
The resistances of 24 oil palm species to pathogens of YZ-4,YZ-6 and YZ-8 were evaluated by the
method of needle inoculation. The results showed that different cultivars behaved different resistances to different
pathogens. RY-2 and RY-6 had higher disease resistance to YZ-4. RY-9,RY-14,RY-15,RY-31,RY-37
and RY-38 showed good resistance to YZ-6,and YZ- 8 showed weak pathogenicity to RY-38. In addition,the
antagonistic activity,production of hydrolytic enzymes and secondary metabolites was carried out on 138 strains
isolated from different habitats( roots,leaves,soil). Results showed that strains produced IAA had the largest
percentage according to 75.36 percent and no strain produced chitinase and Glucanase,the strains producing
protease,cellulase and siderophore were 67.39% ,58.70% and 63.04%,respectively. Further result showed
that AR156 and 3BS3 were proved to have antagonistic activity on YZ-4 and YZ-8,which reached to 13 and 10
scores respectively. HNLE-4-2 and 3BS3 showed well antagonistic activity on YZ-6 and comprehensive score
was 10. The results provide the preliminary research foundation for obtaining high quality of biocontrol bacteria
resources. |
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