| 杨 柳，钟志梅.C3，6- 乙酰化缩氨基硫脲类衍生物抑菌活性研究[J].广东农业科学,2017,44(7):104-111 |
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C3，6- 乙酰化缩氨基硫脲类衍生物抑菌活性研究 |
| Study on antimicrobial activity of C3，6-diacetylated phenyl-thiosemicarbazone-chitosan derivatives |
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| DOI：10.16768/j.issn.1004-874X.2017.07.017 |
| 中文关键词: 壳聚糖 抑菌活性 乙酰化缩氨基硫脲 |
| 英文关键词: chitosan Antimicrobial activity Diacetylated phenyl-thiosemicarbazone |
| 基金项目:内蒙古农业大学校园杰出青年基金（214203025）；国家自然科学基金（21064004，21462030） |
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| 中文摘要: |
| 通过测试10 种壳聚糖C3，6- 乙酰化缩氨基硫脲类衍生物对4 种动物致病细菌和4 种植物致病
真菌的抑菌效果，筛选出几种活性较好的药物前体化合物。采用逐级稀释法，利用酶标仪测定12 个浓度下
药物的OD 值，并计算对动物致病细菌抑菌率，根据抑菌率确定其最小抑菌浓度（MIC）和最小杀菌浓度
（MBC）。采用生长速率法测定衍生物在5 个浓度下对植物致病真菌的抑菌率。结果表明，10 种衍生物对
所有测试菌种的抑菌效果均优于原料壳聚糖，对革兰氏阴性菌的抑菌效果优于革兰氏阳性菌，对大肠杆菌
的最小抑菌浓度和最小杀菌浓度分别为14.80、29.16 μg /mL；所有衍生物在0.05～2 mg/mL 浓度范围内对
测试真菌都有一定的抗菌作用，最大抑菌率为100%。对黄瓜灰霉病菌的抑菌效果低于其他3 种植物致病真
菌。其中C3，6- 乙酰化高分子量壳聚糖苯胺基缩（3，6-DAPTSCZHCS）、C3，6- 乙酰化高分子量壳聚糖对甲
苯胺基缩氨基硫脲（3，6-DA（p-T）TSCZHCS）、C3，6- 乙酰化高分子量壳聚糖对硝基苯胺基缩氨基硫脲
（3，6-DA（p-NP）TSCZHCS）、C3，6- 乙酰化低分子量壳聚糖邻氯苯胺基缩氨基硫脲（3，6-DA（o-CP）
TSCZLCS）、C3，6- 乙酰化低分子量壳聚糖对硝基苯胺基缩氨基硫脲（3，6-DA（p-NP）TSCZLCS）5 种壳
聚糖衍生物对所有测试菌种均有较强的抑菌活性，具有进一步研发的价值。 |
| 英文摘要: |
| By testing the antimicrobial activity of 10 kinds of C3，6-dichloroacetyl phenyl- thiosemicarbazonechitosan
derivatives on four kinds of animal pathogenic bacteria and four kinds of plant pathogenic fungi，several
compounds with better activity were selected as prodrug. By using Stepwise Dilution Method，the OD value of the
derivatives in 12 concentrations was determined by the Microplate Reader，and the minimum inhibitory concentration
（MIC） and the minimum bactericidal concentration（ MBC） were determined according to the inhibitory rate of
bacteria. A Mycelium Growth Rate Method was used to determine the inhibitory rate of derivatives on plant pathogenic
fungi at five concentrations. The results showed that the antimicrobial effects of 10 kinds of derivatives on all tested
strains were better than that of raw chitosan. The antibacterial effect of gram negative bacteria was better than that
of Gram-positive bacteria. The minimum inhibitory concentration（ MIC） and minimum bactericidal concentration
（MBC） of the derivatives against Escherichia coli were 14.80 μg/mL and 29.16 μg/mL respectively. All of the
derivatives had a definite antifungal effect on fungi in the concentration range of 0.05 mg/mL-2 mg/mL，with a
maximum inhibition index of 100%. The antifungal activity of F. oxysporum f. sp. cucumerinum was lower than that of
other three kinds of plant pathogenic fungi. 3，6-DAPTSCZHCS，3，6-DA（p-T）TSCZHCS，3，6-DA（p-NP） TSCZHCS，3，6-DA（o-CP）TSCZLCS，3，6-DA（p-NP）TSCZLCS had strong antimicrobial activity to all test
strains，and had the value for further research and development. |
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