| 陈 静1，何吉祥1，黄 龙1，吴本丽1，孙和权2，宋光同1，陈贵生3，王晓健4.3 个中华鳖群体遗传多样性的微卫星标记分析[J].广东农业科学,2017,44(7):141-146 |
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3 个中华鳖群体遗传多样性的微卫星标记分析 |
| Microsatellite marker analysis of genetic diversity in three populations of Trionyx sinensis |
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| DOI：10.16768/j.issn.1004-874X.2017.07.022 |
| 中文关键词: 中华鳖 武昌湖 荧光SSR 遗传多样性 遗传分化 |
| 英文关键词: Trionyx sinensis Wuchang Lake fluorescent SSR marker genetic diversity genetic differentiation |
| 基金项目:安徽省科技攻关项目（1604a0702018）；安庆市科技计划项目；安徽省农业科学院成果推广项目
（16E0505）；安徽省水产产业技术体系( 皖农科［2016］84 号) |
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| 中文摘要: |
| 为了给武昌湖中华鳖的遗传资源保护和利用提供较为准确的基础数据，利用微卫星标记技术，
对中华鳖武昌湖野生群体和2 个养殖群体进行遗传多样性分析。结果显示：武昌湖鳖群体、日本鳖群体、黄
沙鳖群体的平均等位基因数（Na）分别为11.5、9.1、7.9，平均有效等位基因数（Ne）分别为6.3、3.7、3.1，
平均观测杂合度（Ho ）分别为 0.5667、0.5222、0.4462，平均期望杂合度（He）分别为0.6987、0.5953、
0.5648，多态信息含量（PIC）分别为 0.6672、0.5551、0.5303，武昌湖群体遗传多样性较高。基于Nei’s 遗
传距离和遗传分化指数显示，目前武昌湖群体中华鳖种质资源保存较好。 |
| 英文摘要: |
| To provide more accurate basic data for the genetic resources of Trionyx sinensis of Wuchang
lake，and protect and utilize their germplasm resources，microsatellite marker technology was used to analyze the
genetic diversity of T. sinensis from wild population of Wuchang lake and two breeding populations as experimental
objects. The results showed that the average number of alleles（ Na）for the three populations were 11.5，9.1 and
7.9 respectively，average effective numbers of alleles（ Ne） were 6.3，3.7 and 3.1 respectively，average observed
heterozygosity （Ho） were 0.5667，0.5222 and 0.4462 respectively，average expected heterozygosity（ He） were
0.6987，0.5953 and 0.5648 respectively and the average polymorphism information content（ PIC） were 0.6672，
0.5551 and 0.5303 respectively，the genetic diversity of Wuchang Lake population was higher，the Nei’s genetic
distance and genetic differentiation showed that the germplasm resources preservation of Wuchang Lake population
was better now. |
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