| 张 帅1，黄嘉玲1，林怡丽1，程 昊2，3.紫外照射对灵芝菌丝体多糖的影响[J].广东农业科学,2017,44(8):26-32 |
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紫外照射对灵芝菌丝体多糖的影响 |
| Effect of ultraviolet radiation on polysaccharides of Ganoderma lucidum mycelia |
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| DOI：10.16768/j.issn.1004-874X.2017.08.005 |
| 中文关键词: 紫外照射 灵芝 菌丝体 多糖 抗氧化 |
| 英文关键词: ultraviolet radiation Ganoderma lucidum mycelia polysaccharides antioxidation |
| 基金项目:广东省大学生创新创业训练计划项目（201610580050）；广西高等学校高水平创新团队及卓越学者计
划项目（2016TZYKF01） |
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| 摘要点击次数: 2189 |
| 全文下载次数: 1000 |
| 中文摘要: |
| 采用紫外照射诱导菌种的方法可使灵芝菌丝体多糖含量增加。首先考察了不同紫外照射时间
和功率对灵芝菌丝体形态、生长速度及多糖含量的影响，发现小功率、短时间的照射有利于菌丝体的生长，
并可提高菌丝体多糖的含量，而大功率、长时间的照射则会对灵芝菌丝体产生相反的影响。然后通过正交
试验，确定了紫外照射提高灵芝菌丝体多糖含量的最优条件，即用20 W 的紫外灯照射灵芝菌种10 min，灵
芝菌丝体多糖含量可达9.57%，比未经紫外照射的灵芝菌丝体多糖含量提高了3.45%。另外，将未紫外照射
和在最优条件下紫外照射的灵芝的菌丝体多糖进行抗氧化能力比较，发现二者清除·OH 和O2
-· 自由基
能力无明显差异。 |
| 英文摘要: |
| The content of polysaccharides from Ganoderma lucidum mycelia can be increased using the method
of ultraviolet（ UV） radiation introduction. The effects of different time and power of UV radiation on morphology，
growth rate，and polysaccharides content of mycelia were firstly investigated. The results showed that UV radiation
with low power and short time was conductive to mycelia growth and increasing polysaccharides content，and the
opposite effects on those occurred under the condition of UV radiation with high power and long time. Then，the
optimum UV radiation condition of increasing polysaccharides content was obtained by orthogonal test，that was，
G. lucidum strains was radiated for 10 min using a UV lamp with 20 W，and the mycelia polysaccharides content
can reach 9.57%，which was 3.45% higher than that of without UV radiation. In addition，the antioxidant ability
of mycelia polysaccharides from G. lucidum radiated by UV under the optimum conditions was compared with that
without UV radiation. The results indicated that ·OH and O2
-· scavenging capacities of the two polysaccharides
were not significantly different. |
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