张少平,徐呈祥,李超群,郑福庆.檀香紫檀苗木炭疽病病原菌分离鉴定[J].广东农业科学,2017,44(8):85-89 |
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檀香紫檀苗木炭疽病病原菌分离鉴定 |
Pathogen isolation and identification of a leaf disease of red sandalwood( Pterocarpus santalinus) seedlings |
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DOI:10.16768/j.issn.1004-874X.2017.08.014 |
中文关键词: 檀香紫檀 叶部病害 病原菌 分离鉴定 胶孢炭疽菌 |
英文关键词: red sandalwood( Pterocarpus santalinus) leaf disease pathogen isolation and identification Colletotrichum gloeosporioides |
基金项目:国家自然科学基金(31270674);肇庆学院创新强校重点研究平台(CQ201607) |
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中文摘要: |
在檀香紫檀引种繁育中,发现其温室中的苗木冬春时节发生一种较严重的叶部病害,但病害名
称、病原菌种类未见报道。以感染该病害的18 月龄苗木为试材,分离纯化后获得病原菌株,采用形态学与
分子生物学相结合的方法对其进行鉴定。根据分离到的该菌株菌落形态特征、分生孢子形态特征及病原菌
rDNA-ITS 序列测试分析结果,确定分离到的菌株为胶孢炭疽菌(Colletotrichum gloeosporioides);致病性试
验结果表明,胶孢炭疽菌是导致檀香紫檀叶片该种病害的病原菌,但属非典型炭疽病。讨论了檀香紫檀苗
木炭疽病的防控。 |
英文摘要: |
In the introduction and propagation of red sandalwood( Pterocarpus santalinus),we found a serious
leaf disease of its seedlings in winter and spring seasons,but the name of the disease and its pathogen species
have not been reported. The pathogen isolated from infected leaves of 18-month-old seedlings was identified as
Colletotrichum gloeosporioides by morphological characteristics of colony and conidium,and analysis results of
rDNA- internal transcribed spacer sequence( ITS) of the strain. Pathogenicity test results further confirmed that
C. gloeosporioides was the pathogen responsible for the infected leaves symptoms of red sandalwood,however,the
disease belongs to an atypical anthracnose. Control of the leaf diseases of red sandalwood seedlings was discussed. |
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