| 郭晋杰，陈景堂.玉米籽粒容重QTL 定位[J].广东农业科学,2017,44(9):8-14 |
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玉米籽粒容重QTL 定位 |
| QTL mapping for test weight of maize kernel |
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| DOI：10.16768/j.issn.1004-874X.2017.09.002 |
| 中文关键词: 玉米 容重 SSR 标记 QTL 定位 遗传连锁图谱 |
| 英文关键词: maize test weight SSR makers QTL mapping linkage map |
| 基金项目:河北省高等学校科学技术研究重点项目（ZD 2 0 1 7 0 3 7）；国家重点研发计划项目（2 0 1 6 Y F D
0101204-3；2017YFD0101202）；河北省现代农业技术体系玉米创新团队项目（HBCT2013020204） |
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| 摘要点击次数: 2122 |
| 全文下载次数: 716 |
| 中文摘要: |
| 以优良玉米自交系农系531（NX531）和航天诱变后的农系531（M-NX531）为亲本组配
F2∶3 群体，2012 年在邯郸农科院试验田对亲本和定位群体进行夏播，收获后考种测得籽粒容重性状表型
数据，利用SSR 标记构建此群体的遗传图谱，采用复完备区间作图法（ICIM），对玉米籽粒容重性状进
行QTL 定位，并对其加性效应和上位性效应进行分析。研究结果表明，构建的遗传图谱总的遗传长度为
1 179.8 cM，约占玉米全基因组的85.76%，标记间的平均距离为11.91 cM。通过定位分析，检测到容重的加
性QTLs 共3 个，分别位于第1、6、8 条染色体上，共解释20.29% 的表型遗传变异；检测到容重的上位性
QTLs 共3 对，涉及6 个位点，分布于第1、3、4、10 共4 条染色体上，并解释67.08% 的表型遗传变异。除了
加性效应和显性效应外，上位性效应也是容重的重要遗传基础。 |
| 英文摘要: |
| In the study，we used the elite inbred line Nongxi531（ NX531） and the space mutation inbred line
Nongxi531（ M-NX531） as the parents to building F2∶3 populations to investigating the bulk density traits，and
planted the parents and F2:3 populations in Handan academy of agricultural sciences in the summer of 2012，and
measured bulk density traits after harvesting. Using the SSR marker to constructed the genetic map of this population.
Used the complete complex interval mapping method（ ICIM） for QTL mapping，and evaluated the additive and
epistatic effects of QTLs. The results showed that the total length of the genetic map was 1 179.8 cM，which occupied
85.76% of the whole maize genome，and the average distance between the markers was 11.91 cM. We detected 3
additive QTLs through the QTL mapping analysis，which were located on 1st，6th，8th chromosomes，and explained
20.29%phenotypic variation；detected 3 epistatic QTLs，which involving 6 sites，located in 1st，3th，4th and 10th
chromosomes，expanded the phenotypic variation in 67.08%. These results indicated that except the additive and
dominant effects，epistasis effect was also an important genetic basis for bulk density of maize. |
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