| 黄勋和1，2，余哲琪1，翁茁先1，2，3，温金星4，李威娜1，2，钟 鸣1，2，唐亚东2，5，钟福生1，2.基于线粒体DNA D-loop 序列的五华三黄鸡遗传多样性及其品种起源研究[J].广东农业科学,2017,44(9):106-113 |
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基于线粒体DNA D-loop 序列的五华三黄鸡遗传多样性及其品种起源研究 |
| Genetic diversity and breed origin of Wuhua Yellow chicken inferred from mitochondrial DNA D-loopsequences |
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| DOI：10.16768/j.issn.1004-874X.2017.09.017 |
| 中文关键词: 五华三黄鸡 线粒体DNA D-loop 遗传多样性 系统进化 |
| 英文关键词: Wuhua Yellow chicken mitochondrial DNA D-loop genetic diversity phylogeny |
| 基金项目:广东省自然科学基金（2014A030307018）；广东省公益研究与能力建设项目（2015A020208020，
2016A030303068）；嘉应学院省市共建重点建设项目（嘉院[2017]27 号） |
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| 中文摘要: |
| 通过PCR 产物直接测序法对五华三黄鸡保种群120 个个体进行线粒体DNA（mtDNA）D-loop
序列测定，研究该品种的遗传多样性和系统进化。结果显示，五华三黄鸡保留着较高水平的遗传多样性。
mtDNA D-loop 的C+G 含量（42.5%）低于T+A 含量（57.5%），核苷酸多样性和核苷酸差异均数分别为
0.01298 ± 0.00051 和6.750。共检测到33 个突变位点，均为转换突变，包括23 个T>C 转换和10 个A>G 转
换，占分析位点的6.35%。定义了32 种单倍型，其中独享型单倍型19 个，单倍型多样性为0.913 ± 0.012，
遗传距离为0.013 ± 0.003。单倍型类群主要分布在进化枝A、B、C 和E，其中B 为优势单倍型类群。系统
进化分析和进化枝地理分布特征表明，五华三黄鸡有4 个母系来源，可能受北方家鸡南迁的影响。 |
| 英文摘要: |
| For depicting genetic diversity and phylogeny of Wuhua Yellow chicken，one hundred and twenty
samples were used to directly sequence using PCR products of mitochondrial DNA D-loop. The results showed that
the Wuhua Yellow chicken obtained high levels of genetic diversity，with nucleotide diversity，average number of
nucleotide differences and haplotype diversity for 0.01298 ± 0.00051，6.750 and 0.913 ± 0.012，respectively.
The content of C++G（ 42.5%） was lower than T+A（ 57.5%）. A total of 33 mutation sites were detected，including
23 T to C and 10 A to G transitions，accounted for 6.35% of the analyzed sequences. Thirty-two haplotypes were defined，with 19 exclusive haplotypes and 13 shared haloptypes. The haplotype diversity and genetic distance were
0.913±0.012 and 0.013±0.003，respectively. These haplotypes were mainly distributed in clades A，B，C and E，
and B was the dominated haplogroup. Phylogenetic analysis and geographical distribution indicated that there were
four maternal origins to Wuhua Yellow chicken，which was influenced to some extent，by the gene flow of domestic
chicken from northern China. |
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