吴华俊1,2,陈敬祥1,林 同1.松墨天牛14-3-3ζ 基因的鉴定及生物信息学分析[J].广东农业科学,2017,44(10):70-76 |
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松墨天牛14-3-3ζ 基因的鉴定及生物信息学分析 |
Identification and bioinformatics analysis of 14-3-3ζ gene from Monochamus alternatus |
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DOI:10.16768/j.issn.1004-874X.2017.10.012 |
中文关键词: 松墨天牛 14-3-3ζ 基因 同源比对 系统发育树 生物信息学 |
英文关键词: Monochamus alternatus 14-3-3ζ gene homologous alignment phylogenetic tree bioinformatics |
基金项目:国家自然科学基金(31470653);广东省自然科学基金(2015A030313416) |
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中文摘要: |
为探究松墨天牛(Monochamus alternatus)14-3-3ζ 基因特性,通过构建cDNA 文库,从松墨
天牛cDNA 文库中克隆到14-3-3ζ 基因,命名为Ma14-3-3ζ,GenBank 登录号为KT970073.1。克隆得到的
Ma14-3-3ζ cDNA 全长为902 bp,其中开放阅读框为747 bp,编码248 个氨基酸。推测的Ma14-3-3ζ 蛋白等
电点为4.73,分子量为28.16 ku。Ma14-3-3ζ 的氨基酸序列与大黄粉虫(Tenebrio molitor)同源性最高、为
98%,与褐飞虱(Nilaparvata lugens)等12 种昆虫同源性在95%~90% 之间。系统发育树表明松墨天牛与
大黄粉虫在同一分枝上。 |
英文摘要: |
In order to elucidate the characteristics of Monochamus alternatus,the 14-3-3ζ gene from M.
alternatus called Ma14-3-3ζ was cloned by using cDNA library construction,GenBank(KT970073.1). The
cloned cDNA of Ma14-3-3ζ is 902 bp in length,which contains a 747 bp open reading frame( ORF) encoding 248
amino acids with a calculated molecular mass of 28.16 KD and an isoelectric point of 4.73. The results also indicate
that Ma14-3-3ζ show the highest homology with Tenebrio molitor( 98%),and has 95%-90% amino acid sequence
homology with the 14-3-3ζ from 12 species of insects such as Nilaparvata lugens,M. alternatus and T. molitor on the
same branch in the phylogenetic tree based on insect 14-3-3ζ analysis. |
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