| 吴 霄1，李 果1，敖 政1，石俊松2，蔡更元1，刘德武1，吴珍芳1，李紫聪1.过表达H3K9me3 去甲基化酶对猪克隆胚胎体外发育效率的影响[J].广东农业科学,2017,44(10):96-101 |
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过表达H3K9me3 去甲基化酶对猪克隆胚胎体外发育效率的影响 |
| Effects of overexpression of H3K9me3 demethylase on the in vitro developmental efficiency of clonedporcine embryos |
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| DOI：10.16768/j.issn.1004-874X.2017.10.016 |
| 中文关键词: 猪 克隆胚胎 H3K9me3 KDM4B KDM4D |
| 英文关键词: porcine cloned embryos H3K9me3 KDM4B KDM4D |
| 基金项目:广东省科技计划项目（2016B020233006，2016A020210074）；国家自然科学基金（31772554） |
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| 中文摘要: |
| 为了探索过表达组蛋白H3K9me3 去甲基化酶KDM4B/KDM4D 对猪克隆胚胎体外发育效率的
影响，研究构建了鼠源KDM4B/KDM4D 表达载体，通过体外转录获得KDM4B/KDM4D mRNA 并将其分别显
微注射至猪克隆胚胎。结果显示两组注射的克隆胚胎的H3K9me3 水平、卵裂率和囊胚率与对照克隆胚胎无
显著差异，但两组注射的克隆胚胎的H3K9me3 去甲基化酶表达水平显著高于无注射的对照克隆胚胎，且注
射KDM4B mRNA 的克隆胚胎的囊胚细胞数显著高于注射KDM4D mRNA 的克隆胚胎及对照克隆胚胎，表明
过表达组蛋白H3K9me3 去甲基化酶KDM4B 可以显著提高猪克隆胚胎的体外发育效率。 |
| 英文摘要: |
| To explore the effects of overexpression of histone H3K9me3 demethylases KDM4B/KDM4D on the
in vitro developmental efficiency of porcine cloned embryos，KDM4B/KDM4D expression vectors were constructed，
and KDM4B/KDM4D mRNA were obtained by in vitro transcription and were microinjected into cloned pig embryos，
respectively. The results show that injected cloned embryos have a similar H3K9me3 level，cleavage rate and
blastocyst rate with non-injected cloned embryos，but have a significantly higher KDM4B/KDM4D expression level
than non-injected control cloned embryo（ P<0.05），and cloned embryos injected with KDM4B mRNA have a
significantly higher total number of cells per blastocyst than non-injected control cloned embryos（ P<0.05）. These
results suggest that overexpression of histone H3K9me3 demethylases KDM4B can significantly improve the in vitro
developmentral rate of cloned porcine embryos. |
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