刘小飞1,廖飞雄2,李冬梅1,刘晓荣1.基于SRAP 标记的白鹤芋属资源鉴定与亲缘关系分析[J].广东农业科学,2017,44(11):26-31 |
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基于SRAP 标记的白鹤芋属资源鉴定与亲缘关系分析 |
Genetic relationship analysis and cultivar identification of Spathiphyllum using SRAP marker |
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DOI:10.16768/j.issn.1004-874X.2017.11.005 |
中文关键词: 白鹤芋属 SRAP 标记 遗传多样性 聚类分析 |
英文关键词: Spathiphyllum SRAP marker genetic diversity cluster analysis |
基金项目:广东省科技计划项目(2015B070701016) |
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中文摘要: |
利用SRAP 标记对白鹤芋属23 份样品进行遗传多样性分析,30 个引物组合共扩增出343 个位
点,其中多态性位点为214 个,多态性比率达62%。通过 NTSYS 软件计算得到的样品间遗传相似系数介于
0.36~0.98,显示白鹤芋属资源间存在一定的遗传差异;根据SRAP 扩增结果,利用UPGMA 法构建树状聚
类图,聚类结果将23 份样品分为3 组。从分子水平分析白鹤芋属种质的遗传多样性和亲缘关系,为白鹤芋
属种质资源的有效利用和开发提供依据。 |
英文摘要: |
The sequence related amplified polymorphism(SRAP)marker was used to analyze genetic
diversity of 23 samples of Spathiphyllum. Thirty primer pairs generated 343 bands,214 bands(62%)of which
were polymorphic ones. Revealed by NTSYS software,the SM coefficient of genetic similarity ranged from 0.36
to 0.98,which indicated that genetic basis was relatively abundance among the 23 Spathiphyllum resources. A
dendrogram was constructed based on SRAP data using UPGMA cluster method. The 23 samples of Spathiphyllum
were classified into 3 major groups. The genetic diversity and relationships of Spathiphyllum resources were studied
at molecular level for providing new molecular evidences of utilization and exploitation of germplasm resources of
Spathiphyllum. |
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