文章摘要
刘小飞1,廖飞雄2,李冬梅1,刘晓荣1.基于SRAP 标记的白鹤芋属资源鉴定与亲缘关系分析[J].广东农业科学,2017,44(11):26-31
查看全文    HTML 基于SRAP 标记的白鹤芋属资源鉴定与亲缘关系分析
Genetic relationship analysis and cultivar identification of Spathiphyllum using SRAP marker
  
DOI:10.16768/j.issn.1004-874X.2017.11.005
中文关键词: 白鹤芋属  SRAP 标记  遗传多样性  聚类分析
英文关键词: Spathiphyllum  SRAP marker  genetic diversity  cluster analysis
基金项目:广东省科技计划项目(2015B070701016)
作者单位
刘小飞1,廖飞雄2,李冬梅1,刘晓荣1 1. 广东省农业科学院环境园艺研究所/ 广东省园林花卉种质创新综合利用重点实验室 广东 广州 5106402. 华南农业大学林学与风景园林学院广东 广州 510642 
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中文摘要:
      利用SRAP 标记对白鹤芋属23 份样品进行遗传多样性分析,30 个引物组合共扩增出343 个位 点,其中多态性位点为214 个,多态性比率达62%。通过 NTSYS 软件计算得到的样品间遗传相似系数介于 0.36~0.98,显示白鹤芋属资源间存在一定的遗传差异;根据SRAP 扩增结果,利用UPGMA 法构建树状聚 类图,聚类结果将23 份样品分为3 组。从分子水平分析白鹤芋属种质的遗传多样性和亲缘关系,为白鹤芋 属种质资源的有效利用和开发提供依据。
英文摘要:
      The sequence related amplified polymorphism(SRAP)marker was used to analyze genetic diversity of 23 samples of Spathiphyllum. Thirty primer pairs generated 343 bands,214 bands(62%)of which were polymorphic ones. Revealed by NTSYS software,the SM coefficient of genetic similarity ranged from 0.36 to 0.98,which indicated that genetic basis was relatively abundance among the 23 Spathiphyllum resources. A dendrogram was constructed based on SRAP data using UPGMA cluster method. The 23 samples of Spathiphyllum were classified into 3 major groups. The genetic diversity and relationships of Spathiphyllum resources were studied at molecular level for providing new molecular evidences of utilization and exploitation of germplasm resources of Spathiphyllum.
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