| 宋培勇，曾伯平，肖仲久.两株耐高温酵母菌的鉴定及其特性研究[J].广东农业科学,2017,44(12):79-85 |
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两株耐高温酵母菌的鉴定及其特性研究 |
| Identification and charateristics of two thermotolerant yeasts |
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| DOI：10.16768/j.issn.1004-874X.2017.12.013 |
| 中文关键词: 酒糟堆放土壤 耐高温酵母 筛选 鉴定 系统发育分析 耐性测试 |
| 英文关键词: soil of distiller's grains thermotolerant yeast screening identification phylogenenetic analysis tolerance test |
| 基金项目:贵州省科技厅联合基金（黔科合J 字LKZS[2012]25 号）；贵州省科技创新人才团队项目（黔科合人才
团队[2012]4004） |
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| 中文摘要: |
| 用传统微生物学分离培养方法，从特殊自然环境中筛选耐高温酵母，从形态学、生理生化特征
并结合26S rDNA D1/D2 区序列比对及系统发育分析对目的菌株进行鉴定，用常规方法对其耐热、耐酸、耐
糖和耐乙醇性以及乙醇发酵性能进行测试。结果显示，从常年堆放茅台酒糟的土壤中获得的2 株耐高温酵
母，经鉴定分别为Y1 东方伊萨酵母（Issatchenkia orientalis）、Y2 库德毕赤酵母（Pichia kudriavzevii）。耐性
测试表明Y1、Y2 均能在45℃正常生长，但在46、47℃生长不良，菌落出现异常；初始糖浓度达25% 时出
现抑制；初始乙醇浓度分别达8%、10% 时，Y1、Y2 生长受到抑制；最适pH=4。在30、45℃温度条件下，Y1
的乙醇产量分别为3.8% 和5.1%，Y2 分别为8.7% 和9.8%，说明Y2 的耐乙醇性和乙醇发酵性能较Y1 优，
具有用于同步糖化发酵的潜能。 |
| 英文摘要: |
| In order to screen thermotolerant yeasts from special natural environment for ethanol fermentation，
we carried out this study. After obtaining the isolates with traditional microbiological methods，we performed
idenfication for the isolates according to morphological，physiological and biochemistrial characteristics，integrated
26S rDNA D1/D2 section sequences comparison and phylogenenitic analysis. Furthermore，we investigated the
thermotolerance，acidfastness，sugar tolerance，ethanol tolerance and ethanol yield of the isolates by rutine methods.
We obtained two thermotolerant yeasts from the soils for Moutai wine lees stacking，named Y1 and Y2. The Y1 and
Y2 were classified to Issatchenkia orientalis and Pichia kudriavzevii，respectively. The tolerance test results showed
that isolates Y1 and Y2 both could survive at 45℃，whereas they showed poor growth and abnormal colonies when
exceeded 45℃. They showed growth inhibility when initial sugar concentration was up to 25%，and also the same
when initial ethanol concentration was up to 8% and 10% for Y1 and Y2. The optimal pH for them was 4. The ethanol
yields of Y1 and Y2 were 3.8% and 8.7% at 30℃，and 5.1% and 9.8% at 45℃，respectively. The ethanol tolerance
and fermentation capabilty under the two temperature of 30℃ and 45℃ of Y2 was more excellent than Y1. So we
deduced that the isolate yeast Y1 maybe used in simultaneous saccharification and fermentation. |
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