| 谭 琳1，周兆禧1，王甲水1，马伏宁1，康由发2.海南山竹果实不同部位多酚提取物抗氧化活性、乙酰胆碱酯酶和α- 葡萄糖苷酶抑制活性研究[J].广东农业科学,2018,45(1):102-107 |
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海南山竹果实不同部位多酚提取物抗氧化活性、乙酰胆碱酯酶和α- 葡萄糖苷酶抑制活性研究 |
| Antioxidant activity, acetylcholine esterase and α-Glucosidase inhibitory activity of the poly-phenolextractives from peel and pulp of Garcinia mangostana fruit grown in Hainan province |
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| DOI：10.16768/j.issn.1004-874X.2018.01.017 |
| 中文关键词: 山竹 果实多酚 抗氧化活性 自由基清除活性 乙酰胆碱酯酶抑制活性 α- 葡萄糖苷酶抑
制活性 |
| 英文关键词: Garcinia mangostana fruit polyphenols antioxidant activity radical scavenging activity acetylcholine esterase inhibitory activity α-glucosidase inhibitory activity |
| 基金项目:中国热带农业科学院海口实验站科研启动项目（HKZKY140204）；中国热带农业科学院基本科研业务
费（1630092017010） |
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| 摘要点击次数: 2771 |
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| 中文摘要: |
| 了解海南山竹果皮和果肉的多酚含量及其抗氧化活性、乙酰胆碱酯酶抑制活性和α- 葡萄糖
苷酶抑制活性，为引导消费、开发利用奠定基础。以采自海南五指山的山竹果实为材料，用95% 的乙醇提
取山竹果皮和果肉多酚，采用福林- 酚法测定总酚含量。对获得的山竹果皮和果肉多酚提取物进行羟自由
基（·OH）、DPPH 自由基、ABTS 自由基清除率以及乙酰胆碱酯酶抑制活性和α- 葡萄糖苷酶抑制活性测
定。结果表明，山竹果皮多酚含量很高，达21.36 mg/g（干重），对·OH、DPPH 自由基和ABTS+· 均具有
良好的清除活性，且呈现浓度依赖性，IC50 分别为37.39、41.34、41.37 μg/mL；山竹果皮多酚对乙酰胆碱酯
酶和α- 葡萄糖苷酶也具有很强的抑制活性，且呈现浓度依赖性，IC50 分别为29.46、7 μg/mL；山竹果肉多
酚含量较低，为0.388 mg/g（干重），清除DPPH 自由基、ABTS+· 和·OH 活性较弱，但表现出浓度依赖效
应。山竹果肉多酚对α- 葡萄糖苷酶具有中等程度的抑制活性（IC50 为 2.24 mg/mL），对乙酰胆碱酯酶抑制
活性较弱。山竹果皮多酚具有良好的抗氧化性、乙酰胆碱酯酶抑制活性和α- 葡萄糖苷酶抑制活性；山竹
果肉多酚具有中等程度的α- 葡萄糖苷酶抑制活性，一定的抗氧化性和乙酰胆碱酯酶抑制活性。 |
| 英文摘要: |
| The total phenolics were extracted from the peel and pulp of Garcinia mangostana fruit with 95%
ethanol，and were subjected to quantification using Folin phenol method. The antioxidant activities of the peel and
pulp extracts were evaluated by using Hydroxyl，DPPH and ABTS radical method，as well the acetylcholine esterase
and α-glucosidase inhibitory activity were assayed. The results suggested that the peel extract contained a very high
level of total phenolics（ 21.36 mg/g DW） with the high capacity to scavenge DPPH，ABTS and hydroxyl radical
in a concentration-dependent manner. The IC50 were 37.39，41.34，41.37 μg/mL，respectively. It also exhibited the strong inhibitory activity against acetylcholine esterase and α-Glucosidase in a concentration-dependent
manner with IC50 of 29.46 and 7 μg/mL，respectively. Unlike those from peel，the extracts from pulp contained
much lower level of total phenolics（ 0.388 mg/g DW）. The antioxidant activity and inhibitory activity against
acetylcholine esterase were also found to be weaker in the extracts from the pulp when compared with those from
peel，which displayed modest α-glucosidase inhibitory activity with IC50 of 2.24 mg/mL. It was concluded that
the poly-phenols from the peel of Garcinia mangostana fruit had strong antioxidant potential and α-glucosidase
inhibitory activity as well as acetylcholine esterase inhibitory activity，whereas，those from the pulp indicated the
modest level of activities. |
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