| 张 萍1，王 森1，付国赞2，蒋建林1，唐 艳1.顶花板凳果愈伤组织和芽诱导培养研究[J].广东农业科学,2018,45(2):42-46 |
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顶花板凳果愈伤组织和芽诱导培养研究 |
| Induction culture of callus and bud in Pachysandra terminalis |
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| DOI：10.16768/j.issn.1004-874X.2018.02.008 |
| 中文关键词: 顶花板凳果 组织培养 消毒方式 激素配比 光照条件 |
| 英文关键词: Pachysandra terminalis tissue culture disinfecting methods hormone concentration light
condition |
| 基金项目: |
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| 摘要点击次数: 2318 |
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| 中文摘要: |
| 为快速繁殖顶花板凳果优良品种，筛选出顶花板凳果愈伤组织和芽诱导的最佳培养条件，以顶
花板凳果当年生鲜嫩带芽茎段为试材，研究了不同采样时间、植体消毒方式、激素配比、是否光照处理等培
养条件对顶花板凳果愈伤组织和芽的诱导的影响。结果表明：5 月是顶花板凳果最佳采样季节，其外植体的
愈伤组织和芽诱导率高于其他月份、为44%；以仅用0.1％HgCl2 处理5 min 的消毒效果最好，污染率及死亡
率均较低，分别为11%、22%；NAA 0.5 mg/L + 6-BA 2.0 mg/L 为最佳愈伤组织和芽诱导培养的激素浓度及配
比，诱导率高达47%；以光照培养的效果最佳，诱导率为60%。 |
| 英文摘要: |
| In order to rapidly propagate elite varieties of Pachysandra terminalis and screen out optimal culture
conditions for callus and bud induction ，we studied the effects of different culture conditions on induction of callus
and bud in P. terminalis including different sampling time，disinfecting methods of explants，hormone concentration
andlight treatment，taking annual tender stem with lateral buds from P. terminalis as explants. The results showed that
the best sampling season was May which provided acallus induction and bud induction rate of explants of 44% . Better
disinfection effect could be achieved by disinfecting with 0.1% HgCl2 for 6 min，of which the pollution rate and the
death rate was 11% and 22% respectively. The optimal concentration of hormone for inducing medium of callus and
buds was 0.5 mg/L NAA+2.0 mg/L 6-BA，with an induction rate of 47%. Light culture is better than dark culture，
with an induction rate of 60%. |
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