章帅文,杨 勇,刘 群,吴志明,李昆太.拮抗植物病原真菌Streptomyces corchorusii AUH-1 的分离与鉴定[J].广东农业科学,2018,45(2):103-108 |
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拮抗植物病原真菌Streptomyces corchorusii AUH-1 的分离与鉴定 |
Isolation and identification of Streptomyces corchorusii AUH-1 with antagonistic activity against plant pathogenic fungi |
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DOI:10.16768/j.issn.1004-874X.2018.02.017 |
中文关键词: 植物病原真菌 西瓜枯萎病菌 分离鉴定 抑菌率 黄麻链霉菌AUH-1 |
英文关键词: phytopathogenic fungi Fusarium oxysporum f. sp. niveum isolation and identification inhibition
rate Streptomyces corchorusii AUH-1 |
基金项目:国家自然科学基金(31760546);江西省教育厅科技项目(GJJ160389);江西农业大学大学生创新创
业计划项目(201610410022);江西省青年科学家培养对象计划项目(20142BCB23025) |
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中文摘要: |
为了获得对植物病原真菌具有抑制作用的拮抗菌株,以西瓜枯萎病菌为指示菌,采用平板对峙
试验进行植物病原真菌拮抗菌的分离筛选。从南昌、赣州、鹰潭、萍乡、武夷山、石家庄等地采集的26 个不
同类型土样中,分离筛选到1 株对西瓜枯萎病菌具有良好抑制作用的菌株AUH-1,其抑菌率达到36.15%。
根据菌体形态学观察、生理生化特征以及16S rDNA 序列分析,对拮抗菌进行菌种鉴定,结果表明该菌株为
黄麻链霉菌(Streptomyces corchorusii),并命名为黄麻链霉菌AUH-1。 |
英文摘要: |
In order to screen antagonistic bacterium which could inhibit the growth of phytopathogenic fungi,
the antagonistic strain was isolated by screen with Fusarium oxysporum f. sp. niveum as indicator and then using dual
culture. An antagonistic bacterium AUH-1 against F. oxysporum f. sp. niveum was isolated from 26 different types of
soil samples collected from Nanchang,Ganzhou,Yingtan,Pingxiang,Wuyishan and Shijiazhuang,etc.,with the
inhibition rate of 36.15%. Based on morphological characteristics,physiological and biochemical characteristics,
and 16S rDNA sequence analysis,the strain was preliminarily identified as Streptomyces corchorusii and named as S.
corchorusii AUH-1 here. |
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