| 邓雪萍1，杨 博2.一株小叶榕抗菌内生细菌的分离、筛选和鉴定[J].广东农业科学,2018,45(2):109-115 |
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一株小叶榕抗菌内生细菌的分离、筛选和鉴定 |
| Separating，screening and identification of an antimicrobial endophytic bacterium from Ficus microphylla L.f. |
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| DOI：10.16768/j.issn.1004-874X.2018.02.018 |
| 中文关键词: 内生细菌 抗菌活性 筛选 鉴定 小叶榕 |
| 英文关键词: endophytic bacteria antibacterial activity screening identification Ficus microcarpalla L.f. |
| 基金项目:清远职业技术学院科学技术研究项目（ZK12006） |
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| 中文摘要: |
| 从小叶榕茎段和叶片中分离、筛选和鉴定抗菌活性内生细菌，以开发生物防治菌剂。采用组织
块法和组织匀浆法分离内生菌，杯碟法和菌落生长速率法检测内生细菌的抗菌活性，通过培养特征和菌体
形态观察、生理生化特性试验、16S rDNA 序列测定和系统发育树的构建鉴定内生菌。结果表明，从小叶榕
植物样本中分离得到的一株内生细菌QYPT-B01 的发酵滤液对铜绿假单胞菌、大肠埃希菌和黑曲霉有较强
的抑制作用，经鉴定该菌株为枯草芽孢杆菌（Bacillus subtilis），今后可进一步研究该内生菌发酵液中活性
物质的组分及对植物病原菌的拮抗作用。 |
| 英文摘要: |
| This study was done to screen and identify the endophytes with antibacterial activity from Ficus
microcarpa L.f. in order to develop biocontrol agents. Endophytes were isolated by tissue block method and tissue
homogenate method. The bacteriostatic activity of endophytic bacteria was determined by cup dish method and colony
growth rate method. Identifing the endophytic bacteria through the strain characteristics and bacteria morphology
observation，physiological and biochemical characteristic test，determination of 16S rDNA gene sequences，and
construction of phylogenetic tree. The results showed that one of the endophytes named QYPT-B01 had antibacterial
activity. The fermentation filtrate of strain QYPT-B01 had strong bacteriostatic effect on Pseudomonas aeruginosa，
Escherichia coli and Aspergillus niger. The composition of active substances in fermentation broth and its antagonistic
effect on plant pathogenic bacteria could be further studied in the future. |
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