| 王 成，余以刚，张 青，余祥雄，梁泽明.蛴螬抗菌肽的制备及其稳定性研究[J].广东农业科学,2018,45(2):116-122 |
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蛴螬抗菌肽的制备及其稳定性研究 |
| Preparation and stability studies of grub antimicrobial peptides |
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| DOI：10.16768/j.issn.1004-874X.2018.02.019 |
| 中文关键词: 蛴螬 抗菌肽 工艺优化 抑菌活性 酶解 稳定性 |
| 英文关键词: grub antimicrobial peptide optimization antimicrobial activity hydrolysis stability |
| 基金项目:广东省科技计划项目（2016B090918103） |
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| 摘要点击次数: 2388 |
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| 中文摘要: |
| 使用酶解法制备对金黄色葡萄球菌和大肠杆菌都有抑制活性的小分子抗菌肽，以解决滥用抗
生素饲料添加剂导致的抗药性和药物残留问题。实验通过单因素分析法和正交实验法，以加酶量、酶解时
间、料液比和酶解pH 4 个因素，优化制备蛴螬抗菌肽的条件。结果表明，胰蛋白酶为最适合用于制备
蛴螬抗菌肽的蛋白酶，制备抗菌肽的最佳酶解条件为：加酶量50.00 ku/g，酶解时间90 min，料液比1∶
10（g/mL）和酶解pH 7.50。酶解产物对金黄色葡萄球菌和大肠杆菌的抑制作用最强，抑菌圈直径分别为
11.50、8.70 mm。稳定性实验结果表明，蛴螬抗菌肽的最佳作用pH 为6.00，高温环境下具有稳定的抑菌效
果，对于不同的实验菌，盐浓度对抗菌肽的抑菌效果影响不同；蛴螬抗菌肽具有较好的胰蛋白酶水解稳定
性，但不具有胃蛋白酶水解稳定性。制备得到的蛴螬蛋白酶解产物有抗菌效果，可以在农业、医药、食品、
畜牧业等领域发挥重要作用。 |
| 英文摘要: |
| In this study，grub antimicrobial peptides were prepared to solve the antibiotic resistance and drug
residue problem caused by the abuse of traditional antibiotics and feed additives. Enzyme hydrolysis method was used
to produce small molecular antimicrobial peptides which showed strong inhibitory activity against both Staphylococcus
aureus（ S. A） and Escherichia coli（ E. coli）. The results showed that，trypsin was found to be suitable for the
preparation of antimicrobial peptides. Orthogonal experiment was used to determine the optimum experimental
conditions for the preparation of antimicrobial peptides using trypsin. Orthgonal experiment showed that when
protease adding was 50.00 ku/g，hydrolysis duration was 90min，the ratio of substance to liquid was 1∶10，pH was
7.50，the diameter of antimicrobial circle reached the greatest value against S. A and E. coli which is 11.5 mm and
8.7 mm respectively. The stability tests showed that the optimum pH was 6.00，and it had a stable antibacterial effect
in high temperature environment. The effect of salt concentration on antimicrobial activity was different for different
experimental bacteria. The antibacterial peptide of grubs had good resistance against trypsin hydrolysis，but had no
stability to pepsin hydrolysis. These results indicated that grub antimicrobial peptides had strong antimicrobial activity
and could be used in the area of agriculture，medicine and food industry. |
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