| 刘 露，孙连立，裴运林，聂艳峰，张黎兴，龙京洲.不同干燥方式对金线莲黄酮体外活性影响的研究[J].广东农业科学,2018,45(3):107-113 |
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不同干燥方式对金线莲黄酮体外活性影响的研究 |
| Effects of different drying methods on the activity of total flavonoids of Anoectochilus roxburghii（wall.） in vitro |
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| DOI：10.16768/j.issn.1004-874X.2018.03.017 |
| 中文关键词: 金线莲黄酮 真空冷冻干燥 恒温鼓风干燥 抗氧化 黑色素合成 酪氨酸酶活性 |
| 英文关键词: Anoectochilus roxburghii flavonoids vacuum freeze-drying air-drying antioxidant capacity tyrosinase activity melanin |
| 基金项目:广州市科技计划项目（201604016114） |
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| 中文摘要: |
| 采用真空冷冻干燥、恒温鼓风干燥两种方法，开展了其对金线莲黄酮体外抗氧化能力、抑制酪
氨酸酶活性、抑制黑色素合成的影响研究。结果表明：两种干燥方式处理金线莲黄酮含量具有显著差异，
冷冻干燥有利于提高金线莲黄酮含量和抑制黑色素、酪氨酸酶活性成分的保留，其黄酮含量高达24.55（±
0.28）mg/g；体外抗氧化实验显示在0.2～5.0 mg/mL 浓度范围内金线莲黄酮的抗氧化能力与浓度成正比，且
真空冷冻干燥金线莲黄酮（FDAF）抗氧化能力均显著高于恒温鼓风干燥金线莲黄酮（ADAF），在5 mg/mL
时其对DPPH 清除率为60.16（±0.005）%，还原力为0.73（±0.015），总抗氧化能力为1.21（±0.006）
mmol/mL（Fe2+）；对人黑色素瘤A375 细胞实验结果显示FDAF 对酪氨酸酶活性抑制率显著高于ADAF，但
对黑色素合成抑制作用显著低于ADAF，这可能与所含不同黄酮单体有关。两种干燥方式的金线莲干样黄
酮提取率、体外抗氧化能力、及其对黑色素合成、酪氨酸酶活性的抑制作用均低于鲜样。研究结果对金线莲
在功能食品、化妆品等领域开发应用提供理论依据。 |
| 英文摘要: |
| The present paper studied the effects of two drying technologies，vacuum freeze-drying and airdrying，
on the antioxidant capacity，tyrosinase activity and melanin biosynthesis of Anoectochilus roxburghii in
vitro. The results showed that the content of flavonoids was significantly different in A. roxburghii with two drying
methods. The amount of flavonoids was as high as 24.55（ ±0.28） mg/g in the vacuum freeze-drying method. In
vitro experiments also showed that the antioxidation activity of flavonoids of A. roxburghii within the concentration
range of 0.2-5.0 mg/mL was proportional to the flavonoids concentration using vacuum freeze-drying. However，the
activity of flavonoids reduced significantly as affected by air-drying method. The antioxidant capacity of flavonoids
in freeze-drying Anoectochilus roxburghii（ FDAF） was significantly higher than that of the air-drying（ ADAF） at
the concentration of 5.0 mg/mL with a DPPH clearance rate of 60.16（ ±0.005）%，a reducing power of 0.73（ ±
0.015），and a total antioxidant capacity of 1.21（ ±0.006） mmol Fe2+/mL. The results of human melanoma A375
cells showed that the inhibition rate of tyrosinase activity by FDAF was significantly higher than that of ADAF，but
the inhibitory effect on melanin production was significantly lower than that of ADAF，which may be related to the
different flavonoid monomers. The experimental results demonstrated that the inhibition rate of tyrosinase activity by FDAF was significantly higher than that of ADAF，but the inhibition of melanin synthesis was significantly lower
than that of ADAF in the human melanona A375 cell，which might result from difference of flavonoid monomers. These
results provided theoretical basis for Anoectochilus Roxburghii as a valuable ingredient for the foods，cosmetics and
pharmaceutical industries. |
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