| 路 梅1，凌丹燕2，马锡文1，郭卫东1.蓝莓枝枯病病原菌分离鉴定及孢子萌发条件研究[J].广东农业科学,2018,45(4):114-120 |
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蓝莓枝枯病病原菌分离鉴定及孢子萌发条件研究 |
| Separation，identification and spore germination conditions of the pathogen causing shoot blight on blueberry（Vaccinium spp.） |
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| DOI：10.16768/j.issn.1004-874X.2018.04.017 |
| 中文关键词: 蓝莓 枝枯病 棒状拟盘多毛孢菌 rDNA-ITS 序列分析 分离鉴定 孢子萌发 |
| 英文关键词: blueberry（Vaccinium spp.） shoot blight Pestalotiopsis clavispora rDNA-ITS sequence
analysis isolation and identification spore germination |
| 基金项目:浙江省公益技术应用研究计划项目（2015C32080） |
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| 中文摘要: |
| 2016 年在浙江省蓝莓种植基地发现原因不明的幼苗木整株枯死现象，为明确其致病菌，通过
组织分离法和单孢培养法进行菌株的分离纯化，通过科赫氏法则进行分离菌的致病性检测，运用常规形态
学和rDNA-ITS 序列分析相结合的方法进行病原菌分类鉴定，同时使用载玻片法进行病原菌孢子萌发条
件研究。结果表明：蓝莓整株植株枯死是由枝枯病所致，纯化菌株ZA1 为该病病原真菌。其菌落圆形，白
色；分生孢子呈纺锤形，被4 个真隔膜分成5 个细胞，中间细胞呈褐色，两端细胞无色。结合rDNA-ITS 序
列分析，菌株ZA1 被鉴定为棒状拟盘多毛孢菌（Pestalotiopsis clavispora）。菌株ZA1 孢子萌发的最适温度为
30℃；对悬浮液酸碱度的适应性较广，pH 3～12 时孢子均可萌发；光照和复合碳源均有助于孢子萌发。 |
| 英文摘要: |
| In 2016，some individuals of blueberry in Zhejiang plant base were dead in the seedling stage. In
this study，to identify the pathogenic bacteria，some methods including tissue separation，single spore purification，
Koch's postulate，conventional morphological and molecular biology were used. The factors affecting spore germination
were also explored，including temperature，pH value，illumination condition，medium component and so on. Results
showed that the dead plants were caused by shoot blight，which was induced by ZA1. The colony of pathogen ZA1
was round and white，and the conidium was spindle-shaped，divided into five cells by four true septums，and the
intermediate cell was brown，the cells at both ends were colorless. The pathogen ZA1 was identified as Pestalotiopsis
clavispora by morphological observation and sequence analysis of rDNA-ITS. Meanwhile，the optimum temperature
for spore germination was 30℃，otherwise，lower temperature（ below 20℃） affected germination rate significantly.
The adaptability of strain ZA1 spores was very broad，whose pH value could change among pH 3-12. And both light
and complex carbon sources were beneficial for spore germination. |
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