| 王苗苗1，2，于 杰2，钟 云1，姜 波1，吴 波1，闫化学1.外源NO 诱导下MAPK 基因在采后柑橘果实抗绿霉病中的表达分析[J].广东农业科学,2018,45(4):121-131 |
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外源NO 诱导下MAPK 基因在采后柑橘果实抗绿霉病中的表达分析 |
| Expression analysis of the MAPK genes in exogenous NO induced disease resistance to Penicillium digitatumin the postharvest citrus fruits |
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| DOI：10.16768/j.issn.1004-874X.2018.04.018 |
| 中文关键词: MAPKs 基因 抗病反应 一氧化氮 绿霉病菌 柑橘果实 |
| 英文关键词: MAPK genes disease resistance nitric oxide Penicillium digitatum citrus fruit |
| 基金项目:广东省科技计划项目（2017A070702005，2016B020201006，2014B020203003） |
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| 摘要点击次数: 2290 |
| 全文下载次数: 693 |
| 中文摘要: |
| 一氧化氮（NO）是植物逆境响应的重要信号分子，能够诱导激活丝裂原激活蛋白激酶
（MAPK）的活性。为了分析外源NO 诱导下MAPK 基因在采后柑橘果实抗绿霉病中的表达情况，以埃及
糖橙（Citrus Sinensis）果实为材料，使用硝普钠（SNP）溶液处理果实，统计接种柑橘绿霉病菌后果实的发
病率和病斑面积，荧光定量PCR 检测果实中MAPK 基因的相对表达量和变化趋势。结果表明：外源NO 能
够降低试验期间柑橘果实绿霉病的发病率；果皮中MAPK 基因的表达受外源NO 诱导；CsMAP1、CsMAP15
和CsMAP13-like 基因在NO 处理1 h 即受诱导表达，CsMAP、CsMAP9、CsMAP9-like、CsMAP13-like、
CsMAP19-like 和CsMAP20-like 在NO 处理4 h 达到表达高峰，CsMAP4-like、CsMAP7 和CsMAPNTF3 在
NO 处理12 h 达到表达高峰，CsMAP1、CsMAP15、CsMAPMMK1 和CsMAPMMK2 分别在NO 处理4 h 和12
h 有两个表达高峰。说明外源NO 提高了柑橘果实对绿霉病的抗性，果皮中MAPK 基因的诱导表达可能在
抗病中发挥作用。 |
| 英文摘要: |
| Nitric oxide（ NO） is an important signal molecule involved in plant defense responses. It also
induces the activation of the mitogen-activated protein kinase（ MAPK）. To study the expression of MAPK genes
in exogenous NO induced disease resistance to Penicillium digitatum in the postharvest citrus fruits，Aijitangcheng
（Citrus Sinensis） fruits were immersed in SNP aqueous solution，the disease incidence and lesion diameter were
measured and recorded during the experiment. Time-course changes in the expression of MAPK genes in response
of citrus fruits peel to P. digitatum inoculation were quantified by using real-time quantitative PCR（ RT-qPCR）.
The results show that exogenous NO can effectively reduce the green mold incidence and lesion diameter of citrus
fruit during storage. The expression of MAPK genes was induced by exogenous NO in the citrus fruits peel. CsMAP1，
CsMAP15 and CsMAP13-like expression were induced rapidly by NO after 1 h of NO treatment. CsMAP，CsMAP9， CsMAP9-like，CsMAP13-like，CsMAP19-like and CsMAP20-like expression level were induced during the early
stages of storage and peaked at 4 h after SNP treatment. CsMAP4-like，CsMAP7，CsMAPNTF3 reached their
highest expression level at 12 h after NO treatment. CsMAP1，CsMAP15，CsMAPMMK1 and CsMAPMMK2 showed
high expression level at 4 h and 12 h after NO treatment. The results suggest that the expression of MAPK genes
in the citrus fruits peel may involve in the resistance of the citrus fruits against P. digitatum by treating with
exogenous NO. |
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