王永强,王 珺.锤状中鼓藻培养条件的优化[J].广东农业科学,2018,45(4):140-145 |
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锤状中鼓藻培养条件的优化 |
Optimization of cultural conditions of Bellerochea malleus |
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DOI:10.16768/j.issn.1004-874X.2018.04.020 |
中文关键词: 锤状中鼓藻 生态因子 营养盐 生长 |
英文关键词: Bellerochea Malleus ecological factors nutritions growth |
基金项目:海南省重点研发计划项目(ZDYF2016087);国家星火计划项目(2015GA800003) |
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中文摘要: |
通过单因子和正交试验,研究了温度、光照、盐度等生态因子及氮、磷、铁、硅、钼、维生
素B1、维生素B12 和维生素H 等营养元素对锤状中鼓藻〔Bellerochea malleus(Bright-well)Van Heurek〕
生长繁殖的影响。结果表明:锤状中鼓藻生长的适宜温度为15~35℃,最适温度为30℃;适宜光照为
1 000~8 000 lx,最适光照为4 000 lx;适宜盐度为5~50,最适盐度为35。筛选出锤状中鼓藻的培养液配
方为每升天然海水中加入NaNO3-N 20.0 mg、NaH2PO4-P 1.0 mg、FeSO4-Fe 0.1 mg、Na2SiO3-Si 15.0 mg、
Na2MoO4 0.02 mg、VB1 1.0 mg 和VH 0.05 mg。 |
英文摘要: |
The paper studied effects of temperature,illumination intensity,salinity and nutrients on the growth
and reproduction of Bellerochea malleus by one-factor experiment and orthogonal experiments.Results showed that
the suitable water temperature was 15-35℃ and the optimal water temperature was 30℃.The suitable and optimal
illumination intensity were respectively 1 000-8 000 lx and 4 000 lx.The suitable salinity was 5-50 and the optimal
salinity was 35.The optimal prescription for culture solution was as follows:NaNO3-N 20.0 mg/L,NaH2PO4-P 1.0
mg/L,FeSO4-Fe 0.1 mg/L,Na2SiO3-Si 15.0 mg/L,Na2MoO4 0.02 mg/L,VB1 1.0 mg/L and VH 0.05 mg/L. |
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