| 岳少康，谢 鑫，陈 滢，李绪彦，边少敏.lncRNA 基因At3NC026490 启动子克隆与功能分析[J].广东农业科学,2018,45(5):52-57 |
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lncRNA 基因At3NC026490 启动子克隆与功能分析 |
| Cloning and functional analysis of the promoter of a lncRNA-coding gene At3NC026490 |
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| DOI：10.16768/j.issn.1004-874X.2018.05.010 |
| 中文关键词: 拟南芥 lncRNA At3NC026490 启动子 GUS 分析 |
| 英文关键词: Arabidopsis lncRNA At3NC026490 promoter GUS analysis |
| 基金项目:国家自然科学基金（31370340）；国家级大学生创新创业训练计划项目（2017A82374） |
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| 中文摘要: |
| lncRNAs 是一类长度大于200 nt 的非编码RNA，它们通过调控基因表达或直接影响蛋白功能，
在植物生长发育与胁迫响应过程中发挥重要作用。At3NC026490 是拟南芥中的一个lncRNA，目前尚未见该
lncRNA 功能方面的报道。从启动子角度对At3NC026490 的功能进行探索，首先利用生物信息学手段分析
At3NC026490 启动子的特性，发现该启动子具有响应光、激素、胁迫等作用元件；随后构建At3NC026490 启
动子驱动GUS 基因的表达载体并转入拟南芥中；GUS 染色分析表明转基因株系的根、幼苗、叶等营养组织
以及花器官的花瓣具有明显的GUS 表型，而在不同发育时期的种子中并未观察到GUS 表型，说明该启动子
具有典型的组织驱动特性，表明At3NC026490 基因可能在应答光、激素、胁迫刺激以及特定组织中发挥作
用，该研究为后期深入阐释lncRNA 的功能提供了重要信息和研究思路。 |
| 英文摘要: |
| lncRNAs are a class of noncoding RNAs with more than 200 nt in length，which play important roles
in plant growth and response to stresses through regulating gene expression or directly affecting protein function.
At3NC026490 is a lncRNA in Arabidopsis，and its functions totally remain unknown. In the study，the functional
roles of At3NC026490 were investigated in the aspect of its promoter. Firstly，bioinformatic analysis indicated that
the promoter of At3NC026490 gene contains cis-elements responding to light，hormone and stresses. Subsequently，
the promoter was constructed to drive GUS gene，and transformed into Arabidopsis. Transgenic plants showed GUS
phenotype on vegetative tissues（ seedling，root，leave） and sepal of flower，whereas on GUS phenotype was not
observed on seeds at differently developmental stages，suggesting that the promoter showed tissue-driven feature. The
findings implied that the At3NC026490 gene might play roles in response to light，hormone and stresses as well as
specific tissues，which provided important information for further studying the functions of At3NC026490. |
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