| 李向菲，刘小琼，方 芳，王文玉，吴 炫，裴 斐.酶解米糠制备低聚糖阿魏酸酯及其抗氧化活性分析[J].广东农业科学,2018,45(6):90-95 |
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酶解米糠制备低聚糖阿魏酸酯及其抗氧化活性分析 |
| Enzymatic preparation and antioxidation of freuloylated oligosaccharides from rice bran |
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| DOI：10.16768/j.issn.1004-874X.2018.06.015 |
| 中文关键词: 木聚糖酶 酶解条件 超氧阴离子自由基 羟自由基 还原力 |
| 英文关键词: xylanase enzymolysis condition superoxide anion radical hydroxy radical reducing power |
| 基金项目:江苏省高等学校自然科学研究面上项目（17KJB550005） |
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| 中文摘要: |
| 以米糠为原料，采用酶水解法从米糠中提取低聚糖阿魏酸酯（FOs）。以FOs 的提取量为指
标，研究酶浓度、温度、pH 和反应时间等4 个提取因素对酶解效果的影响。结果表明，当料液比为1∶25
（g/mL）时，酶解最佳条件为：酶浓度1 800 U/mL，温度45℃，pH 4.0，酶解时间24 h；此时，米糠中所提取
FOs 的平均含量可达0.93 mmol/L。在此基础上，通过DPPH、羟自由基、超氧阴离子清除率和还原力测定，
结果表明FOs 具有较强的抗氧化活性，其中，FOs 对超氧阴离子的清除能力和还原力与剂量呈线性相关。 |
| 英文摘要: |
| In this study，feruloylated oligosaccharides（FOs）was prepared from rice bran by enzymatic
hydrolysis. Factors of enzyme concentration，enzymolysis temperature，enzymolysis pH，and reaction time on the
hydrolysis results were investigated with FOs production as index. The results showed that the optimal enzymolysis
conditions were as follows，the ratio of liquid to solid 1∶25（g/mL），enzyme concentration 1 800 U/mL，
temperature 45℃，pH 4.0 and reaction time 24 h. The average contents of FOs reached 0.93 mmol/L. On this basis，
the results of DPPH，hydroxyl radical and superoxide anion clearance showed that FOs had strong antioxidant activity.
The effect of FOs on scavenging and reducing power of superoxide anion was linearly correlated with the dose. |
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