| 柴喜荣，林 洁，杨 暹，康云艳.黄瓜品种抗疫病鉴定及其防御相关酶系研究[J].广东农业科学,2018,45(7):16-21 |
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黄瓜品种抗疫病鉴定及其防御相关酶系研究 |
| Identification of resistance of cucumber seedlings toPhytophthora melonis andrelated defense enzyme activity changes |
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| DOI：10.16768/j.issn.1004-874X.2018.07.003 |
| 中文关键词: 黄瓜 甜瓜疫霉菌 病程相关蛋白 抗病品种筛选 |
| 英文关键词: Cucumis sativus Phytophthora melonis pathogenesis related protein disease resistance screens |
| 基金项目:国家自然科学基金（31301767）；广东省高等学校优秀青年教师培养计划项目（YQ2015028）；广东现代农业产业技术体系专项（2016LM1109） |
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| 中文摘要: |
| 以长春密刺、正源油绿花青大吊瓜、中农20号、抗病二号、早青二号、津优1号、粤秀3号7个黄瓜品种为试材，灌根接种约5×103个/mL甜瓜疫霉（Phytophthora melonis）游动孢子悬浮液，探讨不同品种的抗病性以及防御相关酶活性变化。结果表明：长春密刺、正源油绿花青大吊瓜、抗病二号和粤秀3号病情指数范围的抗性较强，属于中抗品种；中农20号、早青二号和津优1号病情指数范围的抗性较弱，属于感病品种；未发现高抗或免疫品种。未接种条件下，正源油绿花青大吊瓜叶片的过氧化物酶（POD）、多酚氧化酶（PPO）、苯丙氨酸解氨酶（PAL）、β-1,3-葡聚糖酶（GLU）活性均显著高于感病品种中农20号；灌根接种疫霉菌游动孢子悬浮液后，中抗品种叶片POD、PAL活性显著增强，其余相关酶活性无显著变化；感病品种黄瓜幼苗叶片POD、PPO、PAL、GLU活性均显著增强，且增幅高于中抗品种。综上所述，甜瓜疫霉侵染后，抗性较强的黄瓜品种叶片防御相关酶活性相对稳定，主要通过上调细胞壁合成相关酶活性抵御病原菌的侵染；相反，感病品种叶片中防御相关酶活性显著被诱导。 |
| 英文摘要: |
| The aim of our study is to investigate the resistance of different cucumber varieties to Phytophthora melonis, and the changes of related defense enzyme activity by inoculated with about 5×103/mL suspension of P. melonis. Seven cucumber cultivars were selected for disease resistance screening, Changchunmici, Huaqingdadiaogua, Kangbing NO.2, Zaoqing NO.2, Zhongnong NO.20, Jinyou NO.1 and Yuexiu NO.3. The results showed that four middle resistant varieties were found, respectively Changchunmici, Huaqingdadiaogua, Kangbing NO.2 and Yuexiu NO.3, the other varieties showed susceptible to P. melonis, no immune and high resistant varieties were found. On uninoculated conditions, middle resistant variety Huaqingdadiaogua had higher activities of peroxidase （POD）, polyphenoloxidase（PPO）, phenylalnine ammonialyase（PAL）and β-1, 3-glucanase（GLU）than susceptible variety Zhongnong NO.20. After inoculation, only POD and PAL activities increased greatly in middle resistant variety, and other related defense enzyme activities kept stable. However, activities of POD, PPO, PAL and GLU all increased significantly in susceptible variety, and the increase degree was higher than that in middle resistant variety. In concluded, middle resistant variety mainly induced cell wall synthesis related enzyme, and thus prevented further invasion and spreading of the pathogen, however, related defense enzyme activities in susceptible variety increased greater. |
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