| 张欣欣1，曲高阳，杨 莹，庄晓萌，张 岚.近红外光谱技术建立镜鲤新鲜度定量预测模型[J].广东农业科学,2018,45(9):121-128 |
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近红外光谱技术建立镜鲤新鲜度定量预测模型 |
| Isolation and identification of Orf virus ORFV/GD-QY/01 strain |
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| DOI：10.16768/j.issn.1004-874X.2018.09.020 |
| 中文关键词: 近红外光谱 镜鲤 挥发性盐基氮 硫代巴比妥酸 偏最小二乘法 BP 人工神经网络 |
| 英文关键词: NIRS mirror carp volatile base nitrogen thiobarbituric acid PLS BP artificial neural network |
| 基金项目:吉林省大学生创新创业训练计划项目（B2016306） |
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| 摘要点击次数: 1763 |
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| 中文摘要: |
| 以镜鲤为研究对象，利用近红外光谱技术和化学计量法采集、测定相关指标，并应用偏最
小二乘法(PLS)、偏最小二乘法和BP人工神经网络两种方法经比较优化模型。经过鱼肉样品光谱的扫描及
pH值、TVB-N（挥发性盐基氮）值、TBA（硫代巴比妥酸）值的测定，在21种预处理下，确定最佳建模方
式、预处理方式和最优波段。经模型优化得知，pH、TVB-N、TBA均在偏最小二乘法中建立的模型最好，
最优预处理方法分别为基线校正和标准正态变量变换、净分析信号、Savitzky-Golay导数和基线校正，最
优波段分别为1 000~1 300 nm和1 700~1 799 nm、1 000~1 200 nm和1 300~1 650 nm、1 000~1 799 nm，并且
pH、TVB-N和TBA的Rc分别为0.9906、0.99865、0.99971，Rp分别为0.6436、0.021357、0.7723，达到了利
用近红外光谱技术对镜鲤新鲜度高效、快捷、无损伤的定量检测预测模型的建立。 |
| 英文摘要: |
| Taking mirror carp as the research object, the near infrared spectroscopy (NIRS) and stoichiometry
were used to collect and determine the related indexes. The partial least squares (PLS), partial least squares (PLS) and
BP artificial neural network were used to optimize the model. The optimum modeling method, pretreatment method
and wave band were determined under 21 pretreatments by scanning the spectrum of fish and pH value, TVB-N (volatile
base nitrogen) value and TBA (thiobarbituric acid) value. The model optimization shows that pH, TVB-N and TBA
are the best models in the partial least squares method. The optimal preprocessing methods are baseline correction
and standard normal variable transformation, net analysis signal, Savitzky-Golay derivative and baseline correction.
The optimal bands respectively are 1000-1300 and 1700-1799 nm, 1000-1200 and 1300-1650 nm, 1000-1799 nm.
The RC values of pH, TVB-N and TBA respectively were 0.9906, 0.99865 and 0.99971, respectively. The Rp values
respectively were 0.6436, 0.021357 and 0.7723. The establishment of a quantitative detection and prediction model
for freshness of mirror carp by near infrared spectroscopy was achieved. |
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