| 罗燕羽，刘绍钦，黄绍力，刘伟光，张木清，黄炽辉.离体培养中利用病菌粗毒素检测香蕉枯萎病抗性研究[J].广东农业科学,2018,45(10):45-49 |
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离体培养中利用病菌粗毒素检测香蕉枯萎病抗性研究 |
| Study on resistance of banana fusarium wilt detected by using crude toxin in vitro culture |
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| DOI：10.16768/j.issn.1004-874X.2018.10.007 |
| 中文关键词: 香蕉枯萎病 粗毒素 离体培养芽 抗病育种 |
| 英文关键词: fusarium wilt of banana crude toxin in vitro culture buds disease resistance breeding |
| 基金项目:广州市科技计划项目（201607010279） |
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| 摘要点击次数: 2101 |
| 全文下载次数: 925 |
| 中文摘要: |
| 在香蕉组织培养过程中，将香蕉枯萎病菌粗毒素添加到组织培养基中，以测定不同品种香蕉对粗毒素的敏感性，并对薄片培养芽用粗毒素进行多次诱变以筛选抗（耐）病突变体。结果表明：供试5个品种香蕉离体培养芽对粗毒素的敏感性程度为农科1号＞粉蕉＞粉杂＞巴西蕉＞东莞大蕉，这与田间真实的品种抗病性不相符合，说明粗毒素虽然对不同抗性的香蕉品种均具有致病作用，但香蕉离体培养芽对粗毒素的敏感性程度与香蕉品种的抗病性无相关关系。巴西蕉和农科1号通过薄片诱导的不定芽对粗毒素的半致死浓度为40.63 mg/L和31.82 mg/L，两个品种香蕉离体培养芽经枯萎病菌粗毒素多次诱变筛选后可获得稳定的耐毒素芽。 |
| 英文摘要: |
| In the process of banana tissue culture, the crude toxin of Fusarium oxysporum f. sp. cubense was added to the tissue culture medium to determine the sensitivity of different varieties of banana towards the crude toxin, and the slice culture buds were subjected to multiple mutagenesis to screen resistant varieties. The results showed that the sensitivities of five varieties of banana in vitro to the toxins was Nongke No.1 > Fen jiao > Fen za > Brazilian banana > Dongguan plantain , which was inconsistent with the disease resistance of the real varieties in the field. It indicated that Fusaric acid had pathogenic effects on banana varieties with different resistance, but the sensitivity of banana in vitro culture to the crude toxin and the resistance of banana varieties had no correlation. The semi-lethal concentration of the crude toxin was 40.63 mg/L and 31.82 mg/L through the slice-induced adventitious buds of Brazilian banana and Nongke No.1, and the stable toxin-resistant buds were obtained through repeated mutagenesis of the crude toxin. |
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