| 樊胜南，喻国辉，陈燕红，黎永坚，陈川雁.枯草芽孢杆菌TR21 可湿性粉剂对香蕉根际土壤微生物的影响[J].广东农业科学,2018,45(10):68-73 |
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枯草芽孢杆菌TR21 可湿性粉剂对香蕉根际土壤微生物的影响 |
| Influence of Bacillus subtilis strain TR21 wettable powder on the banana rhizosphere microorganisms |
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| DOI：10.16768/j.issn.1004-874X.2018.10.011 |
| 中文关键词: 枯草芽孢杆菌 香蕉 根际微生物 生物多样性 丰富度 |
| 英文关键词: Bacillus subtilis banana rhizosphere microorganism bio-diversity abundance |
| 基金项目:广东省公益研究与能力建设项目（2014A020208015）；广东省现代农业产业技术推广体系建设项目（2017LM4178） |
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| 中文摘要: |
| 为了研究枯草芽孢杆菌TR21可湿性粉剂施用对香蕉根际土壤微生物的影响，采集TR21可湿性粉剂处理和清水对照不同种植时期的香蕉根际土样10份，分别提取各土壤总DNA，采用PCR-DGGE、主条带回收、基因克隆和序列测定分析的方法，比较不同处理根际土壤微生物组成差异。结果表明：与未种植香蕉的土样相比，香蕉种植初期，对照土样的物种丰富度S值和多样性指数H值均下降，但经TR21处理的土壤S值增加、H值变动不大；香蕉种植中期，对照土壤的S值和H值上升，达到未种植时的数值，但经TR21处理的土壤S值持续增加、H值下降；随着种植时间的推移直至有香蕉枯萎病发病时，对照和TR21处理土壤的S值持续增加、H值持续下降，但经TR21处理的发病植株和健康植株的根际S值均大于对照、H值均小于对照；对差异条带鉴定及主条带回收和测序分析后均发现，TR21处理后根际土壤的芽孢杆菌增多。说明用枯草芽胞杆菌TR21可湿性粉剂灌根处理香蕉，可以提高香蕉根际土壤微生物的S值，并引起根际芽孢杆菌数量增加。 |
| 英文摘要: |
| In order to study effects of TR21 wettable powder on soil microorganism in banana rhizosphere, ten rhizosphere soil samples from different planting stage treated by TR21 wettable powder and water control were collected, and the total DNA of each soil was extracted respectively, and also, the differences in microbial composition of rhizosphere soil were compared with the methods of PCR-DGGE, main strip recovery, gene cloning and sequencing analysis. The results showed that: Compared with the soil samples without banana planting, both S value (species richness) and H value (diversity index) in the control treatment rhizosphere soil decreased in the early stage of banana planting, but the S value increased and the H value changed little in rhizosphere soil of TR21 treatment.In the middle stage of banana planting, the S value and H value of the control treatment rose to the level of the non planting value, but TR21 treatment's S value increased and its H value decreased. During the later stage, in the rhizosphere soil both treatment of the control and TR21 S value increased and H value decreased continuously. However, the S values of TR21 treatment were greater and the H values were less than that of the control treatment. The number of Bacillus in rhizosphere soil increased after TR21 treatment according to the identification of differential bands and the analysis of main bands recovery and sequencing. Thus, the S value and the number of bacilli in rhizosphere were increased with application of Bacillus subtilis TR21 wettable powder by irrigating the root of bananas. |
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