| 冯迪南 1
，王梦瑶 1
，余成鹏 1
，刘琼光 1，2.马铃薯软腐病菌室内药剂及拮抗细菌筛选[J].广东农业科学,2018,45(12):69-75 |
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马铃薯软腐病菌室内药剂及拮抗细菌筛选 |
| Screening of the agents and antagonistic bacteria to Pectobacterium carotovorum subsp. brasiliense |
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| DOI：10.16768/j.issn.1004-874X.2018.12.012 |
| 中文关键词: 马铃薯细菌性软腐病菌 溴菌腈·壬菌铜 中生菌素 拮抗细菌 粘质沙雷氏菌 生物防治 |
| 英文关键词: Pectobacterium carotovorum subsp. brasiliense bromothalonil·cuppric nonyl phenolsulfonate zhongshengmycin antagonistic bacteria Serratia marcescens biological control |
| 基金项目:华南农业大学省级创新训练项目（201710564010） |
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| 中文摘要: |
| Pectobacterium carotovorum subsp. brasiliense（Pcb）引起的马铃薯细菌性软腐病是马铃薯
重要病害之一，在我国马铃薯产区普遍发生，危害严重，其化学防治和生物防治研究和应用较少。以 Pcb
为指示菌，选择 9 种常用的细菌药剂，通过室内平板试验，筛选出对 Pcb 具有抑菌作用的 4 种药剂，抑
菌效果依次为万胜清（20% 溴菌腈·5% 壬菌铜）、克菌康（3% 中生菌素）、辛菌胺醋酸盐和金霉唑
（1.5% 噻霉酮）；进一步从土壤中分离并获得对 Pcb 有拮抗作用的 5 个拮抗细菌 RFjk1~RFjk5，它们分
别与菠萝泛生菌（Pantoea ananatis）、粘质沙雷氏菌（Serratia marcescens）、变形假单胞菌（Pseudomonas
plecoglossicida）及松鼠葡萄球菌（Staphylococcus sciuri）的 16SrDNA 序列相似性在 99% 以上，鉴定为粘质
沙雷氏菌的拮抗细菌 RFjk2，显示出潜在应用前景，可望为马铃薯细菌性软腐病的化学防治和生物防治奠
定基础。 |
| 英文摘要: |
| Bacterial soft rot of potato, caused by Pectobacterium carotovorum subsp. brasiliense（Pcb）, is
one of the most important diseases in the world. It is widespread and serious in potato producing areas in China and
usually causes huge economic losses. Little investigation has been done on applications of chemical and biological
control to the disease in China. In this study, Pcb was used as the indicator bacteria against nine kinds of chemical
agents to develop effective chemicals on the plate experiment. Four kinds of chemicals, including Wanshengqing（20%
bromothalonil + 5% cuppric nonyl phenolsulfonate）, gramcazole （3% zhongshengmycin）, octyl amine acetate
and Kecocon（1.5% thiazolone）have acquired good antibacterial effect on Pcb respectively. Further investigation
showed that five antagonistic bacterial strains which were isolated from soils have good bacteriostatic activities to
Pcb and were preliminarily identified as Pantoea ananatis, Serratia marcescens, Pseudomonas plecoglossicida and
Staphylococcus sciuri by 16S rDNA, respectively. The antagonistic bacterial strain of RFjk2 which was identified as
Serratia marcescens showed a potential application on biological control of potato soft rot. The results would lay the
foundation for the chemical and biological control to bacterial soft rot of potato. |
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