| 黄 元 1
，刘 涛 1
，陈锦良 1
，王晓虎 1
，陈 晶1
，梁培新 2，黄 武 3，向 华 1.阳江地区某猪场塞内卡病毒抗原和抗体的检测与分析[J].广东农业科学,2018,45(12):107-111 |
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阳江地区某猪场塞内卡病毒抗原和抗体的检测与分析 |
| Detection and analysis of antigen and antibody of Seneca virus from a pig farm in Yangjiang |
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| DOI：10.16768/j.issn.1004-874X.2018.12.018 |
| 中文关键词: 塞内卡病毒 病毒分离 序列分析 抗体中和试验 |
| 英文关键词: Seneca virus A virus isolation sequence analysis antibody neutralization test |
| 基金项目:国家重点研发计划项目（2016YFD0501500）；国家自然科学基金-广东联合基金项目
（U1501213）；广东省科技计划项目（2017A020208034）；广东省农业科学院院长基金（201624） |
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| 中文摘要: |
| 塞内卡病毒是新发现的一种小 RNA 病毒，能使猪产生类似水疱样病变，并引起仔猪死亡。
塞内卡病毒作为外来病原，近几年呈现蔓延趋势。从广东阳江地区某猪场采集病变组织和血清进行检测分
析，针对塞内卡病毒 VP1-2A 基因进行 RT-PCR 扩增，得到特异性的单一条带。对 PCR 产物序列分析表明，
与 2015 年美国毒株 USA/GBI29/2015 的同源性最高、为 99.2%，而与我国 2017 年毒株 CH-HN-2017 的同
源性为 97.1%。将水泡皮处理后接种 PK-15 细胞，盲传 4 代产生显著病变，滴度可达 107 TCID50/0.1mL。
对采集的 16 份血清进行抗体中和试验表明，发病种猪的抗体效价最高达到 1︰4096，9 头种猪全部感染
SVA，发病率为 66.7%，总感染率为 68.8%。 |
| 英文摘要: |
| Seneca virus A(SVA) is a newly discovered picornavirus that produces similar vesicle-like lesions in
pigs and causes death of piglets. In recent years it outbroke and showed a trend of spread . The tissues and serum were
collected from a pig farm in Yangjiang, for testing and analysis. A specific single band was amplified from RT-PCR
of the VP1-2A gene of Seneca virus. Sequence analysis of PCR products showed that this stain was most homologous
（99.2%）with USA/GBI29/2015, a strain from USA in 2015, and showed 97.1% homology with CH-HN-2017, a
stains from China in 2017. The pathological tissue was grinded and the fluid was inoculated to cell line PK-15, and
the cells turned out a significant lesion in the 4 generation, and the titer reached 107 TCID50/0.1mL. The antibody neutralization test of 16 serum samples showed that the highest titer of antibody was 1:4096 in sick breed pigs. All 9
breed pigs were infected with SVA. The incidence of SVA was 66.7% and the total infection rate was 68.8%. |
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