| 段瑞军1，吴朝波 2，3，王 军1，黄圣卓 1，梅文莉 1,戴好富1
，戴好富.海水胁迫对冰菜细胞损伤和有机小分子渗透调节物质积累影响[J].广东农业科学,2019,46(1):21-27 |
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海水胁迫对冰菜细胞损伤和有机小分子渗透调节物质积累影响 |
| Cumulative Effects of Seawater Stress on Cell Damage of Mesembryanthemum crystallinum L. and Small Organic Molecules of Osmoregulation Substances |
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| DOI：10.16768/j.issn.1004-874X.2019.01.004 |
| 中文关键词: 冰菜 MDA 细胞膜透性 可溶性糖 甜菜碱：脯氨酸 |
| 英文关键词: Mesembryanthemum crystallinum L. MDA cell membrane permeability soluble sugar betaine proline. |
| 基金项目:海南省自然科学基金（318MS094）；农业农村部财政专项（NFZX2018）；海南省热带特色高效农业产业专项资金（第一批）（琼财农［2017］1478）；中国热带农业科学院基本科研业务费南药产业技术创新团队项目（17-CXTD-15）；国家现代农业产业技术体系建设专项资金项目（CARS-21） |
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| 中文摘要: |
| 【目的】探究海水胁迫对冰菜细胞损伤和脯氨酸、可溶性糖、甜菜碱积累影响。【方法】以冰菜为材料，
采用盆栽试验研究不同浓度海水（20%、30%、40%、50%、60%）胁迫下冰菜细胞损伤（MDA、细胞膜透性）
和有机小分子渗透调节物质（可溶性糖、甜菜碱、脯氨酸）的变化。【结果】随海水浓度增加，冰菜各级分枝
细胞膜透性增强、可溶性糖含量增加；各级分枝 MDA、甜菜碱含量变化为一级、二级分枝增加，三级分枝则先
增加后减少；各级分枝脯氨酸含量变化均为先增加后减少。海水胁迫与细胞膜透性、可溶性糖含量呈显著正相关。
各有机小分子渗透调节物质中仅可溶性糖与海水浓度和膜脂过氧化呈显著正相关。【结论】随着海水浓度升高，
细胞膜受损伤加重。可溶性糖、甜菜碱、脯氨酸在不同浓度海水和不同生长时期起着重要渗透调节作用，且与
海水胁迫相关性表现为可溶性糖＞甜菜碱＞脯氨酸。 |
| 英文摘要: |
| 【Objective】The objective is to explore the cumulative effects of seawater stress on the cell damage of
Mesembryanthemum crystallinum L., proline, soluble sugar and betaine.【Method】A pot experiment was conducted on M.
crystallinum L. to study the changes of cells damage（MDA and cell membrane permeability） and small organic molecular
osmoregulation substances （soluble sugar, betaine and proline） with seawater stress at different concentrations （20%,
30%, 40%, 50%, 60%）. 【Result】With the increase of seawater concentration: the permeability of cell membranes and
the content of soluble sugar were enhanced in each branch of M. crystallinum L. ;MDA and the content of betaine increased in the first and second branches, and increased and then decreased in tertiary branches; and the content of proline increased and
then decreased in all branches. A significant positive correlation was discovered between seawater stress and the permeability
of cell membranes and the content of soluble sugar. For small organic molecules osmoregulation substances, only the content
of soluble sugar had a significant positive correlation with concentration of seawater and lipid peroxidation of membranes.
【Conclusion】With the increase of seawater concentration, the damage of M. crystallinum L. cell membrane is aggravated.
Soluble sugar, betaine and proline play an important role in osmotic regulation in different seawater concentrations and different
growth stages. Moreover, their correlationwith seawater stress is as follows: soluble sugar > betaine > proline. |
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