| 程立君 1，2，王 磊 1
，吴银梅 1，2，高兴国 1
，师 睿 1
，胡志芳 3.高通量测序技术分析光叶珙桐根际土壤微生物多样性[J].广东农业科学,2019,46(1):43-49 |
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高通量测序技术分析光叶珙桐根际土壤微生物多样性 |
| Analysis on Microbial Diversity in Rhizosphere Soil of Davidia involucrate by High-throughput Sequencing |
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| DOI：10.16768/j.issn.1004-874X.2019.01.007 |
| 中文关键词: 光叶珙桐 细菌 真菌 微生物多样性 |
| 英文关键词: Davidia involucrate bacterial fungi microbial diversity |
| 基金项目:云南省教育厅科学研究基金重大专项（ZD2015018） |
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| 中文摘要: |
| 【目的】通过了解不同地区光叶珙桐根际土壤微生物的种类和多样性差异，为光叶珙桐的引种繁
育提供基础数据和理论依据。【方法】采用高通量测序技术对昭通 3 个地区光叶珙桐根际土壤微生物种类及多
样性进行研究，分析了物种组成和丰度、Alpha 多样性、Beta 多样性和菌群结构。【结果】结果表明，细菌群
落中 α- 变形菌纲 Alphaproteobacteria 最丰富（＞ 17.02%），真菌群落在不同地区存在明显差异，在永善三江口
Pezizomycetes 最丰富（17.01%），彝良小草坝 Archaeorhizomycetes 最丰富（29.79%），镇雄塘房 Sordariomycetes
最丰富（20.77%）；3 个地区中永善三江口、镇雄塘房的光叶珙桐根际土壤微生物种群结构相似性较高，其中
永善三江口的光叶珙桐根际土壤细菌多样性最丰富，镇雄塘房的光叶珙桐根际土壤真菌多样性最丰富。【结论】
3 个地区的光叶珙桐根际土壤微生物菌群都具有较高的生物多样性，但物种组成分布上存在差异。 |
| 英文摘要: |
| 【Objective】The rhizosphere soil of Davidia involucrate in different regions was tested in order to
understand the species and diversity of the rhizosphere microorganisms, and provide basic data and theoretical basis for the
introduction and breeding of D. involucrate. 【Method】High-throughput sequencing was applied to study the microbial
species and diversity of rhizosphere soil of D. involucrate in three regions of Zhaotong, and the species composition and
abundance, Alpha diversity, Beta diversity and microbial community structure were analyzed. 【Result】The results
showed that Alphaproteobacteria was the most abundant in the bacterial community (>17.02%), Fungal community was
obviously different in three regions. Pezizomycetes was the most abundant fungi class (17.01%) in Yongshansanjiangkou,
Archaeorhizomycetes was the most abundant fungi class (29.79%) in Yiliangxiaocaoba, Sordariomycetes was the most
abundant fungi class(20.77%) in Zhenxiongtangfang. Among the three regions, the microbial community structures in
Sanjiangkou, Yongshan and Tangfang, Zhenxiong were highly similar. The rhizosphere soil in Sanjiangkou, Yongshan
reflected the richest bacterial diversity, while that in Tangfang, Zhenxiong mirrored the richest fungal diversity. 【Conclusion】
The microbial community in rhizosphere soil of D. involucrate in the three regions had high biodiversity, but there were
differences in the distribution of species composition. |
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