陈 瑞,张 琪,张婷婷,崔乐怡,杨 蓉,杨 蕾,余之琳,刘 鹏.菊芋浸提液对稗草化感作用的研究[J].广东农业科学,2019,46(2):99-105 |
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菊芋浸提液对稗草化感作用的研究 |
Study on the Allelopathy Effects of Aqueous Extracts from Helianthus tuberosus on Echinochloa crusgalli (L.) Beauv. |
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DOI:10.16768/j.issn.1004-874X.2019.02.015 |
中文关键词: 菊芋 浸出液 化感作用 稗草 作用机理 |
英文关键词: Helianthus tuberosus aqueous extracts allelopathy Echinochloa crusgalli functioning mechanism |
基金项目:国家自然科学基金(41571049);浙江省公益技术应用研究计划项目(2015C32127) |
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中文摘要: |
【目的】通过探索不同品种的菊芋浸提液对稗草的化感影响,以期揭示菊芋浸提液的作用机理
并为其应用于杂草防治提供有力依据。【方法】以稗草种子为试材,进行土培试验,研究徐州和潍坊菊芋不同
器官浸提液对稗草的化感作用,测定稗草根长、芽长、发芽率、抗氧化酶活性、丙二醛(MDA)含量及土壤
碱性磷酸酶、转化酶活性与电导率。【结果】随着浸提液浓度升高,种子发芽率不断降低,最高抑制率达到
52.08%,而根长与芽长的最大降幅分别达到 81.96% 和 19.89%;叶过氧化物酶 (POD)、叶过氧化氢酶(CAT)、
土壤碱性磷酸酶活性随浸提液浓度先升后降,浸提液处理稗草 SOD 活性普遍受抑制。潍坊菊芋浸提液能诱导
稗草积累 MDA,而徐州菊芋浸提液使稗草 MDA 含量先升后降。随着浸提液浓度提高,土壤电导率逐渐升高,
其最大增幅达 180.56%;而转化酶活性随潍坊菊芋提取液浓度升高均表现为促进效应。【结论】菊芋浸提液对
稗草的生长、抗氧化能力、MDA、根系土壤酶活性和电导率都有一定的作用,可用于进行杂草防治。 |
英文摘要: |
【Objective】The allelopathic effects of aqueous extracts from different kinds of Helianthus tuberosus on
barnyard grass were explored in order to reveal the mechanism of aqueous extracts from Helianthus tuberosus and provide
a strong basis for their application in weed control.【Method】With the seeds of Echinochloa crusgalli (L.) Beauv. as
experimental materials, a soil culture experiment was conducted to study the allelopathy of aqueous extracts from different
organs of two H. tuberosus varieties from Xuzhou and Weifang on E. crusgalli. and to determine the root length, length of
bud, germination rate, antioxidant enzyme activity, malondialdehyde (MDA) content of Echinochloa crusgalli (L.) Beauv,
and the alkaline phosphatase, invertase activity and electronical conductivity(EC) of soil.【Result】With the increase of
extract concentration, the germination rate of seeds decreased continuously, the highest inhibition rate reached 52.08%,
and the maximum decrease of root length and length of bud reached 81.96% and 19.89%, respectively. The activity of
POD, CAT and soil alkaline phosphatase increased first and then decreased with the increase of extract concentration
and the SOD activity of E. crusgalli Beauv treated with aqueous extracts was generally inhibited. Aqueous extracts from
Weifang H. tuberosus could induce MDA accumulation in E. crusgalli Beauv, while aqueous extracts from Xuzhou H. tuberosus made MDA content of E.crusgalli Beauv rise first and then drop. With the concentration of the aqueous extracts
increased, the soil conductivity increased gradually and its maximum increase rate was 180.56%. The invertase activity
showed a stimulating effect with the increase of the concentration of aqueous extracts from Weifang H. tuberosus.【Conclusion】
Aqueous extracts from H. tuberosus has certain effects on barnyard grass the growth, antioxidant capacity, MDA, and enzymes
activity and EC in root soil of E. crusgalli Beauv. Therefore, aqueous extracts from H. tuberosus could be applied for weed
control. |
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