| 赵鹏飞 1，2，常明山1，2，罗 辑 2，吴耀军 2，杨 娟 3，杨振德 1.一株寄生曲霉 Q527 对黄野螟的感染作用及生物学特性[J].广东农业科学,2019,46(2):106-112 |
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一株寄生曲霉 Q527 对黄野螟的感染作用及生物学特性 |
| Infection Effect of A Strain of Aspergillus sp. Q527 onHeortiavites soides and Its Biological Characteristics |
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| DOI：10.16768/j.issn.1004-874X.2019.02.016 |
| 中文关键词: entomogenous fungi Heortiavites soides pathogenicity biological characteristics biological control |
| 英文关键词: 虫生真菌 黄野螟 致病性 生物学特性 生物防治 |
| 基金项目:广西林业科技项目（桂林科研［2015］第 3 号） |
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| 中文摘要: |
| 【目的】为了获取更多控虫真菌资源，对从野外的黄野螟死亡幼虫分离得到的一株曲霉菌进行
致病性测定和培养，为培养高致病性生防真菌提供理论依据。【方法】以寄生曲霉 Q527 为试验材料，采用浸
渍法测定了寄生曲霉 Q527 对黄野螟的感染作用，利用菌丝生长速率法和产孢量法对其培养条件及产孢特性
进行初步研究。【结果】寄生曲霉 Q527 对黄野螟幼虫具有良好的感染作用，浸渍法接种 7 d 后校正感染率达
83.3%；寄生曲霉 Q527 生长最适培养基为 PDA 培养基，在此培养基培养 7 d 平均菌落为 78.18 mm；pH 为 8 适
应生长和产孢分别为 78.12 mm 和 2.61×109 个；其不耐高温，在 65℃停止生长和孢子萌发；乳糖作为碳源有效
促进寄生曲霉 Q527 菌丝生长，大小为 37.97 mm；可溶性淀粉作为碳源有效促进寄生曲霉 Q527 产孢，产孢量
为 2.03×109 个；蛋白胨作为氮源有效促进寄生曲霉 Q527 菌丝生长和产孢，分别为 51.06 mm 和 2.96×109 个；
在黑暗状态中生长最好，分别为 78.17 mm 和 2.63×109 个。【结论】寄生曲霉 Q527 对黄野螟具有较强的致死
性且具有生长速度快和适应性强等生物学特性，在黄野螟的生物防治中有潜在的应用价值。 |
| 英文摘要: |
| 【Objective】In order to obtain more resources of insect-controlling fungi, the pathogenicity of an
Aspergillus strain isolated from the dead larvae of Heortiavites soides in the field was determined and cultured to provide
a theoretical basis for the cultivation of highly pathogenic biocontrol fungi.【Method】Aspergillus sp. Q527 was used as
experimental material to determine the infection effect of Aspergillus sp. Q527 on H. soides by impregnation method. The
culture conditions and sporulation characteristics of Aspergillus sp. Q527 were studied by mycelium growth rate method
and sporulation quantity method.【Result】The results showed that the Aspergillus sp. Q527 had a good infection effect
on the larvae of H. soides. The corrected infection rate was 83.3% after inoculation for 7 days, and the optimal medium for the growth of Aspergillus sp. Q527 was PDA medium. The average colony cultured on PDA medium was 78.18mm; the pH
8 was suitable for growth and sporulation, with the size of 78.12mm and sporulation amount of 2.61×109; It could not resist
high temperature and stopped growing and sporing germination at 65 C. Lactose, as a carbon source, effectively promoted the
growth of Aspergillus sp. Q527 mycelium, with the size of 37.97mm; soluble starch, as a carbon source, effectively promoted
Aspergillus sp. Q527 sporulation, with the sporulation amount of 2.03×109; peptone, as a nitrogen source, effectively
promoted the growth and sporulation of Aspergillus sp. Q527 mycelium, with the size of 51.06 mm and the sporulation amount
of 2.96×109 respectively; the best growth condition was in dark state with the colony of 78.17mm and the sporulation amount
of 2.63×109.【Conclusion】Aspergillus nomius Q527 is lethal, fast-growing and adaptable to H. soides, and has potential
application value in biological control of H. soides. |
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