| 范俊甫 1
，李军生 1
，李荫展 2，王倩倩 1
，黄国霞1
，阎柳娟 1.5 种呋喃香豆素分子潜在风险分析[J].广东农业科学,2019,46(2):113-119 |
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5 种呋喃香豆素分子潜在风险分析 |
| Analysis on the Potential Risks of Five Furocoumarin Molecules |
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| DOI：10.16768/j.issn.1004-874X.2019.02.017 |
| 中文关键词: 呋喃香豆素分子 共振散射光谱法 相互作用 DNA 结合饱和值 潜在风险分析 |
| 英文关键词: furocoumarin molecules resonance light scattering interaction DNA binding saturation value potentialrisk analysis |
| 基金项目:广西自然科学基金（2018GXNSFDA281030） |
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| 中文摘要: |
| 【目的】研究 5 种呋喃香豆素分子与 DNA 的相互作用，分析其潜在风险。【方法】通过共振散
射光谱法（RLS）计算呋喃香豆素分子与 DNA 的结合饱和值（DNA BSV），通过 DNA BSV 反映药物分子与
DNA 结合能力以评价其潜在风险。【结果】大肠杆菌 DNA 回收试验结果表明，呋喃香豆素类分子可以或部分
进入大肠杆菌细胞内，并与 DNA 发生相互作用；呋喃香豆素 RLS 信号变化与溴化乙锭十分相似，其进入大肠
杆菌 DNA 的能力由强到弱依次为：异补骨脂素 / 补骨脂素、花椒毒酚、香柠檬烯、花椒毒素。【结论】呋喃香
豆素类分子能够从体外转移到大肠杆菌内部并于其 DNA 发生相互作用，且结合模式可能是嵌入；通过计算各
自的 DNA BSV，推测 5 种呋喃香豆素类化合物潜在风险不大，但仍具有一定风险。 |
| 英文摘要: |
| 【Objective】The objective of the paper was to study the interaction between 5 kinds of furancoumarin
molecules and DNA and analyze their potential risks. 【Methods】The DNA binding saturation value (DNA BSV) of
furanocoumarin molecules binding with DNA was calculated through resonance light scattering (RLS), and the drug
molecules’ability to bind with DNA was reflected by such BSV to evaluate their potential risks.【Resultsv】 The
results of recovery experiments indicated that these furocoumarin molecules could enter or partially enter Escherichia coli
cells and interact with DNA. The changes of RLS signals of furan-coumarin molecules were similar to those of ethidium
bromide; the capacity of furan-coumarin molecules that enter E. coli DNA was ranking as follows: iopsoralen/psoralen ＞
xanthotoxol ＞ bergapten ＞ xanthotoxin.【Conclusion】Furancoumarin molecules could be transferred from external of
E. coli to its internal and interact with their DNA, and the binding pattern may be insertion. It was speculated that the 5
tested furancoumarin molecules showed little potential risks but there was still some by calculating the respective DNA
BSV. |
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