| 程雨飞 1
，朱向涛 1
，季 雯1
，洪尔蔓1
，林 芯 1
，范 贞 2，张俊丽 3.牡丹愈伤组织增殖及褐化研究[J].广东农业科学,2019,46(6):30-36 |
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牡丹愈伤组织增殖及褐化研究 |
| Study on Proliferation and Browning of Peony Callus |
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| DOI：10.16768/j.issn.1004-874X.2019.06.005 |
| 中文关键词: 牡丹 愈伤组织 继代增殖 预培养 褐化 赤霉素 |
| 英文关键词: Paeonia suffruticosa callus subculture multiplication pre-culture browning GA3 |
| 基金项目:国家“863”计划项目（2011AA10020701）；浙江省教育厅项目（Y201223938）；浙江农林大学科研启动项目（2044110008）；国家级大学生创新创业训练计划项目（201813283008） |
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| 中文摘要: |
| 【目的】提高牡丹愈伤组织增殖倍数和质量，减轻增殖过程中的褐化现象，为牡丹愈伤组织增殖
提供途径。【方法】以牡丹胚轴、子叶愈伤组织为材料，对不同添加物组合及浓度、不同预培养时间等方法
手段进行探究。【结果】牡丹胚轴愈伤组织继代增殖最佳培养基组合是 PVP 1.0 g/L+IAA 0.2 mg/L+TDZ 1.0
mg/L+GA3 1.0 mg/L，增殖倍数为 5.75；子叶愈伤组织继代增殖的最佳培养基组合是 PVP 2.0 g/L+IAA 0.5
mg/L+TDZ 0.5 mg/L+GA3 1.0 mg/L，增殖倍数为 5.50。采用预培养 30 d 的外植体，可有效提高增殖倍数并减轻褐化。
1.0 mg/L GA3 有利于抑制愈伤组织增殖过程中的褐化现象，并促进愈伤组织增殖。呈淡黄绿色表面附着絮状物且
质地松软的愈伤组织在继代增殖过程中不易褐化，且中后期体积增殖变化明显。【结论】牡丹愈伤组织增殖中
加入 1.0 mg/L GA3 和采用预培养 30 d 的愈伤组织，可以获得较高的增殖倍数并有效抑制褐化，呈淡黄绿色且质
地松软的愈伤组织是良好的愈伤组织增殖状态。 |
| 英文摘要: |
| 【Objective】 The study was to improve the multiplication times and quality of peony callus to reduce
the browning in the process of proliferation and provide a way for the proliferation of peony callus. 【Methods】 Different
additive combinations and concentrations, different pre-culture time and other methods were explored with peony cotyledon
and hypocotyl callus as materials. 【Results】 The best medium combinations for the multiplication of peony hypocotyl
callus and cotyledon callus are PVP 1.0 mg/L+IAA 0.2 mg/L+TDZ 1.0 mg/L+GA3 1.0 mg/L and PVP 2.0 mg/L+IAA 0.5
mg/L+TDZ 0.5 mg/L+GA3 1.0 mg/L, and the proliferation times are 5.75 and 5.50, respectively. The use of explants that
have been pre-cultured for 30d can effectively increase the proliferation times and reduce browning. GA3 1.0 mg/L is
beneficial to inhibit browning during callus proliferation and promoted callus proliferation. The callus in the form of light
yellowish green floccule on the surface with spongy texture is not prone to brown during subculture multiplication, with the
volume proliferation changing significantly in the middle and late stages. 【Conclusion】 The addition of 1.0 mg/L of GA3
and the use of the callus that has been pre-cultured for 30d in the process of peony callus proliferation can achieve higher proliferation times and effectively inhibit browning. Light yellowish green and spongy callus is a sound condition of callus
proliferation. |
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