| 张小康 1
，张雪丽1
，熊秋芳 1,2.萝卜叶缘裂刻性状分子标记的开发与应用[J].广东农业科学,2019,46(7):17-22 |
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萝卜叶缘裂刻性状分子标记的开发与应用 |
| Development and Application of Molecular Markers for Radish Leaf Edge Cracking Traits |
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| DOI：10.16768/j.issn.1004-874X.2019.07.003 |
| 中文关键词: 萝卜 裂叶 分子标记 AFLP 开发与应用 |
| 英文关键词: radish lobed molecular markers AFLP development and application |
| 基金项目: |
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| 摘要点击次数: 1638 |
| 全文下载次数: 599 |
| 中文摘要: |
| 【目的】明确萝卜裂叶性状的遗传特点，开发与裂叶性状连锁的分子标记。【方法】以萝卜“武
青一号”作为母本，“新洲花叶”作为父本杂交得到 F1，将 F1 分别套袋自交和回交，产生 F2 和 BC1，探讨裂
叶性状的遗传规律。【结果】F1 植株叶型表现为裂叶，BC1（F1ד武青一号”）和 F2 中叶全裂与叶浅裂的植
株分离比分别符合 1∶1 和 3∶1，说明萝卜叶缘裂刻的表型符合显性基因遗传模式，萝卜叶缘缺裂受单个遗传位
点控制。利用 BSA 与 AFLP 技术相结合筛选与萝卜叶缘裂刻基因紧密连锁的分子标记，制备的 SCAR 标记引物
HY-18 与萝卜叶缘裂刻基因紧密连锁，其遗传距离为 3.32 cM。【结论】上述标记的获得为萝卜裂叶性状的分
子标记辅助育种以及裂叶基因的克隆奠定了理论基础。 |
| 英文摘要: |
| 【Objective】The study identifies the genetic characteristics of radish leaf cracking traits and develop a
molecular marker which linked to lobed leaf traits.【Method】The radish variety “WUQING No.1”(as female parent) and
“XINZOUHUAYE” (as the male parent) were used to make the hybrid F1，then self-bred and backcross of F1 were made
to get F2 and BC1. And the genetic characteristics of lobed leaf traits were explored.【Result】The results showed that the
leaf edge of F1 was completely lobed. The separation ratio of leaf edge lobed to non-lobed plants in BC1 and F2 were 1∶1
and 3∶1，indicating that the phenotypic characteristic of radish leaf edge lobed matched with dominant genetic heredity
pattern and was controlled by a single genetic locus.The BSA and AFLP were used to screen the molecular markers which
were closely linked to the lobed leaf edge gene of radish，and the SCAR marker primer HY-18 was closely linked with the
lobed leaf edge gene of radish，with the genetic distance of 3.32 cM.【Conclusion】The obtained markers lay a theoretical
foundation for molecular marker assisted breeding and the cloning of lobed leaf genes. |
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