| 胡蓉花 1
，余成鹏 2，任敏华 2，李小勇3，刘琼光 2，4.烟草青枯病菌噬菌体分离及其生物学特性研究[J].广东农业科学,2019,46(11):78-84 |
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烟草青枯病菌噬菌体分离及其生物学特性研究 |
| Isolation and Biological Characteristics of Tobacco Ralstonia solanacearum Bacteriophage |
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| DOI：10.16768/j.issn.1004-874X.2019.11.011 |
| 中文关键词: 茄科劳尔氏细菌 烟草 噬菌体 温度 紫外光 氯仿 乙醚 |
| 英文关键词: Ralstonia solanacearum tobacco bacteriophage temperature ultraviolet chloroform ether |
| 基金项目:中国烟草总公司江西省公司科技项目（赣烟司〔2016〕81 号） |
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| 中文摘要: |
| 【目的】烟草青枯病是我国南方烟区发生普遍而严重的细菌病害，目前尚未有理想的化学防治药
剂，筛选青枯病菌噬菌体，为作物青枯病的防治提供噬菌体资源。【方法】从江西省吉安市烟田采集土壤样本，
采用双层平板培养分离方法，分离青枯菌噬菌体，进一步比较物理和化学因素对噬菌体活性的影响。【结果】
从吉安烟田土壤中分离获得 6 个青枯菌噬菌体，编号为 PTb7-1、PTb1521、PTb1553、P1Tb1556、P2Tb1556 和
PTb574，其生物学特性存在差异。结果显示，多个噬菌体的最适生长温度约为 35 ℃，致死温度为 55~60 ℃，适
宜酸碱度为 pH 5~9，且对紫外光、乙醚和氯仿敏感。然而，噬菌体 PTb7-1 只适合于酸性环境条件下生长繁殖，
噬菌体 P2Tb1556 对紫外光和乙醚不敏感，但对氯仿敏感。【结论】烟草土壤中具有丰富的青枯菌噬菌体资源，
是筛选噬菌体的材料来源。6 个青枯菌噬菌体生物学特性存在差异，噬菌体 P2Tb1556 对紫外光和乙醚不敏感，
但对氯仿敏感。 |
| 英文摘要: |
| 【Objective】Tobacco bacterial wilt a widespread and devastating bacterial disease in southern tobacco
production area of China, and there is no effective chemical for the control of such disease. The screening of phage will
provide phage resources for the prevention and control of tobacco bacterial wilt in the future.【Method】Soil samples were
collected from the tobacco field of Ji'an City, Jiangxi Province, and the bacterial wilt phages were isolated by double-layer plate
culture and isolation method and the effects of physical and chemical factors on the activity of phage were further compared.
【Result】Six Ralstonia solanacearum bacteriophages were isolated from the soil of tobacco field in Ji'an City, and numbered
as PTb7-1，PTb1521，PTb1553，P1Tb1556，P2Tb1556 and PTb574，among which there were differences in biological
characteristics. The results of biological characteristics research showed that the optimum growth temperature for several
phages was about 35℃ , the lethal temperature was 55-60℃ and the optimum pH was 5-9, and those phages were sensitiveto ultraviolet, ether and chloroform. However, phage PTb7-1 was only suitable to grow and propagate under acidic environment.
Phage P2Tb1556 was not sensitive to ultraviolet and ether, but highly sensitive to chloroform.【Conclusion】There are abundant
phage resources in tobacco soil, which is a good source for phage screening. The biological characteristics of the six phages is
different. P2Tb1556 phage is not sensitive to ultraviolet light and ether, but sensitive to chloroform. |
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