沈会芳,蒲小明,杨祁云,张景欣,孙大元,林壁润.香蕉细菌性软腐病防治药剂筛选试验[J].广东农业科学,2020,47(1):98-104 |
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香蕉细菌性软腐病防治药剂筛选试验 |
Selection of Bactericides for ControllingBacterial Soft Rot of Banana |
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DOI:10.16768/j.issn.1004-874X.2020.01.014 |
中文关键词: 香蕉细菌性软腐病 毒力测定 药效 氧氯化铜 噻菌铜 防治效果 |
英文关键词: bacterial soft rot of banana toxicity determination bactericide effect copper oxychloride thiodiazole copper control effect |
基金项目:广东省科技计划项目(2016B020202003);广东省自然科学基金团队项目(2015A030312002);广州市科技计划项目(201704030120);广东省现代农业产业技术体系绿色发展共性关键技术研发创新团队项目(粤农农函〔2019〕559号) |
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中文摘要: |
【目的】筛选防治香蕉细菌软腐病的防治药剂。【方法】应用最小抑制浓度法、离体叶片法和盆栽
试验研究不同药剂对香蕉细菌性软腐病的防治效果。【结果】硫酸链霉素、青霉素钾和中生菌素对病菌的抑制
能力较强,最小抑制浓度分别为 0.2、3.12、6.25 μg/mL;而王铜(氧氯化铜)和噻菌铜对病菌的抑制能力较弱,
最小抑制浓度分别为 200、400 μg/mL。离体叶片法测定结果表明,王铜、噻菌铜和硫酸链霉素对病害防治效果
最好,有效成分为 400 μg/mL 时,防治效果可达 97.67%、96.34% 和 95.12%。在盆栽试验中,先接种病菌再淋
施药物,30% 王铜悬浮剂 600 倍液和 20% 噻菌铜悬浮剂 500 倍液对香蕉细菌性软腐病的防治效果较好,药后 12 d,
盆栽防治效果分别为 79.37% 和 80.15%;先施药再接种病原菌药后 12 d ,30% 王铜悬浮剂和 20% 噻菌铜悬浮剂
对香蕉细菌性软腐病的防治效果为 87.01% 和 89.23%。【结论】王铜和噻菌铜可作为香蕉细菌性软腐病的防治药剂。 |
英文摘要: |
【Objective】The study was to screen bactericides for controlling soft rot of banana.【Method】The
minimal inhibit concentration, detached leaves and pot experiments were adopted to study the control effects of different
bactericides on soft rot of banana.【Result】Streptomycin sulfate, benzylpenicillin potassium and zhongshengmycin
had strong inhibitory capacity, with minimal inhibitory concentrations of 0.2 μg/mL, 3.12 μg/mlL and 6.25 μg/mL,
respectively. But copper oxychloride and thiodiazole copper had low toxicity to the bacteria, with the minimal inhibitory
concentrations of 200 μg/mL and 400 μg/mL, respectively. According to the determination results of detached leaf
method, the control effects of 400 μg/mL copper oxychloride, thiodiazole-copper and streptomycin sulfate were the best,
with control rates of 97.67%, 96.34% and 95.12%, respectively. When copper oxychloride and thiodiazole copper were
sprayed after being inoculated with pathogen in pot experiments, the control effects of 30% copper oxychloride SC 600
times solution and 20% thiodiazole-copper SC 500 times solution were optimal, with control rates of 79.37% and 80.15% onthe 12th day after spraying. While the two bactericides were sprayed before being inoculated with pathogen in pot experiments,
the control effects of 30% copper oxychloride SC 600 times solution and 20% thiodiazole copper SC 500 times solution were
87.01% and 89.23% on the 12th day after spraying.【Conclusion】Copper oxychloride and thiodiazole copper could be
recommended as green bactericides in controlling soft rot of banana. |
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