| 【Objective】New cassava lines suitable for cultivation in Yunnan were selected to provide a scientific basis for variety approval and production promotion.【Method】Ten new lines of cassava were introduced as experimental materials, and two main cassava varieties of Yunnan Province were used as controls to comprehensively evaluate the main biological characteristics, yield traits, quality and resistance. The variety comparative test was carried out in Longyang District, Menghai County, Yuanmou County, Maguan County of Yunnan Province.【Result】The main stem diameters of 56-7, 58-7 and F124 were higher than those of the control varieties, and those of the other seven new cassava lines were lower than the control varieties. There was no significant difference in plant height among ten new cassava lines. The plant heights of 43-3 and F200 were lower than those of the control varieties, and those of the other lines were higher than the control varieties. The main stem height of 58-7, 43-3, 44-7, 30-8 and F124 was higher than that of the control variety SC205, which showed high branching. The branches of main stems of 56-7 and 02-4 were mostly two branches, and those of the other lines were mostly three branches. The yield per plant of 56-7, 58-7, F200, F124, 02-4 was higher than that of the control varieties, and the yield increase per plant of 56-7 and 58-7 reached a very significant level. The dry matter rate of 58-7, 43-3, 14-19, 30-8, F200 and F124 was higher than that of the control variety GR4. The ten new cassava lines had fewer occurrences of diseases and insect pests in different ecological regions of Yunnan, showing a certain resistance.【Conclusion】Among the ten new cassava lines, the 58-7, F124, F200 and 43-3 showed superior comprehensive performance with good yield increasing effects and excellent quality. Such lines have good prospects for promotion and application in production and production experiments can be conducted in different ecological regions of Yunnan in an appropriate time.